Characterization of bifidobacteria by random DNA amplification
| Autor: | Francine Mondou, Denis Roy, Claude V. Déry, Daniel Vincent |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Bifidobacterium adolescentis Microbiology chemistry.chemical_compound Animals Cluster Analysis Humans Bifidobacterium DNA Primers Genetics Electrophoresis Agar Gel Electronic Data Processing biology Probiotics Actinomycetaceae Reproducibility of Results General Medicine biology.organism_classification Dna amplification RAPD Random Amplified Polymorphic DNA Technique Rats chemistry Genetic marker Food Microbiology Cattle Actinomycetales DNA Food Science Plasmids |
| Zdroj: | International journal of food microbiology. 43(3) |
| ISSN: | 0168-1605 |
| Popis: | RAPD conditions were optimized to generate reproducible banding patterns by testing primers, thermocyclers and overall reproducibility in repeat DNA analysis and separate DNA extractions. Five primers were chosen on the basis of band intensity and distribution (between 2 and 10 bands) which clearly distinguished among strains of Bifidobacterium adolescentis, B. animalis, B. bifidum, B. breve, B. infantis and B. longum. The use of five single-primer reactions under optimized conditions improved the resolution and accuracy of the RAPD method for the characterization of dairy-related bifidobacteria. The results indicated that this method was highly reproducible in repeated analysis. Similarity between bifidobacteria strains was evaluated based on their RAPD profiles. Using a set of five primers, it was demonstrated that it may be possible to distinguish three different species of Bifidobacterium (B. breve, B. bifidum and B. adolescentis), based on similarity of the RAPD profiles to known reference strains. Furthermore, application of the RAPD technique may also be useful and faster, than traditional systematics for placement of industrial strains into specific clusters (either B. longum/infantis or B. animalis/lactis). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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