SLC6 family transporter SNF-10 is required for protease-mediated activation of sperm motility in C. elegans
| Autor: | Gillian M. Stanfield, Conrad A. Chong, Angela Hansen, Brittany A. Duffy, Kristin E. Fenker, J. Paul Norton, Jody M. Hansen, Molly C. Jud |
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| Jazyk: | angličtina |
| Předmět: |
Male
GABA Plasma Membrane Transport Proteins endocrine system Motility Cell motility Biology Article Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Morphogenesis medicine Animals Hermaphroditic Organisms Caenorhabditis elegans Caenorhabditis elegans Proteins Spermatogenesis Molecular Biology Sperm motility reproductive and urinary physiology 030304 developmental biology 0303 health sciences SLC6 transporter Spermatid Spermatozoon urogenital system Reproduction Membrane Proteins Cell Biology biology.organism_classification Spermatozoa Sperm Signaling Transport protein Cell biology medicine.anatomical_structure Mutation Sperm Motility C. elegans Body region 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. (1):171-182 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2014.06.001 |
| Popis: | Motility of sperm is crucial for their directed migration to the egg. The acquisition and modulation of motility are regulated to ensure that sperm move when and where needed, thereby promoting reproductive success. One specific example of this phenomenon occurs during differentiation of the ameboid sperm of Caenorhabditis elegans as they activate from a round spermatid to a mature, crawling spermatozoon. Sperm activation is regulated by redundant pathways to occur at a specific time and place for each sex. Here, we report the identification of the solute carrier 6 (SLC6) transporter protein SNF-10 as a key regulator of C. elegans sperm activation in response to male protease activation signals. We find that SNF-10 is present in sperm and is required for activation by the male but not by the hermaphrodite. Loss of both snf-10 and a hermaphrodite activation factor render sperm completely insensitive to activation. Using in vitro assays, we find that snf-10 mutant sperm show a specific deficit in response to protease treatment but not to other activators. Prior to activation, SNF-10 is present in the plasma membrane, where it represents a strong candidate to receive signals that lead to subcellular morphogenesis. After activation, it shows polarized localization to the cell body region that is dependent on membrane fusions mediated by the dysferlin FER-1. Our discovery of snf-10 offers insight into the mechanisms differentially employed by the two sexes to accomplish the common goal of producing functional sperm, as well as how the physiology of nematode sperm may be regulated to control motility as it is in mammals. |
| Databáze: | OpenAIRE |
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