Production of Exfoliative Toxin A by Staphylococcus aureus Isolated from Mastitic Cow's Milk and Farm Bulk Milk
| Autor: | Takeshi Shimano, Hideo Igarashi, Yuji Hayakawa, Hirofumi Komae, Miyoko Endou, Kyuhei Takeuchi, Michiko Hayashi, Shotaro Takeuchi, Nozomu Hashimoto |
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| Rok vydání: | 1998 |
| Předmět: |
Exfoliative toxin
Staphylococcus aureus Biology medicine.disease_cause Polymerase Chain Reaction Microbiology Mice NIKOLSKY SIGN medicine Animals Humans Exfoliative Toxins Mastitis Bovine Immunosorbent Techniques Electrophoresis Agar Gel Microbial toxins General Veterinary Inoculation food and beverages respiratory system Latex fixation test Exfoliatins Titer Milk cardiovascular system Cattle Female Latex Fixation Tests circulatory and respiratory physiology |
| Zdroj: | Journal of Veterinary Medical Science. 60:1281-1283 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.60.1281 |
| Popis: | The production of exfoliative toxins A and B (ETA and ETB) by Staphylococcus aureus isolated from mastitic cow's milk and farm bulk milk was examined by the reverse passive latex agglutination method (RPLA). ETA was detected in 2 (1.2%) of 162 isolates from mastitic cow's milk and in 1 (0.6%) of 166 isolates from farm bulk milk. RPLA titers of these isolates were much lower than in human isolates. No ETB was detected in any of the isolates tested. These ETA-positive isolates belonged to bovine ecovar. They were non-typable using the international phage set for human strains. When these ETA-positive isolates were subcutaneously inoculated into neonatal mice, general exfoliation of the epidermis accompanied by the so-called Nikolsky sign was not recognized. By the immunoblotting and PCR methods, however, ETA and eta gene were recognized in the ETA-positive isolates from mastitic cow's milk and farm bulk milk. These data suggest that ETA is also produced by bovine isolates of S. aureus, but in smaller quantities. |
| Databáze: | OpenAIRE |
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