Involvement of Opioid System and TRPM8/TRPA1 Channels in the Antinociceptive Effect of Spirulina platensis
| Autor: | Eduarda Gomes Ferrarini, Donatella Cicia, Amanda Vasconcelos, Rafael C. Dutra, Maíra Cola, Raffaele Capasso, Gabriela Bonfanti Vieira, Mariana A. Freitas, Elaine C. D. Gonçalves |
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| Přispěvatelé: | Freitas, M. A., Vasconcelos, A., Goncalves, E. C. D., Ferrarini, E. G., Vieira, G. B., Cicia, D., Cola, M., Capasso, R., Dutra, R. C. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Nociception 0301 basic medicine Antioxidant Narcotic Antagonists medicine.medical_treatment Analgesic TRPV1 TRPM Cation Channels Spirulina platensis (+)-Naloxone Pharmacology Microbiology Biochemistry Article Plant Extract Nociceptive Pain functional food Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine opioid system ionic channel Oral administration Spirulina medicine Animals pain TRPA1 Cation Channel Molecular Biology Spirulina (genus) Analgesics biology Animal Naloxone Plant Extracts Chemistry analgesic biology.organism_classification QR1-502 030104 developmental biology Capsaicin Menthol Spirulina platensi Narcotic Antagonist 030217 neurology & neurosurgery |
| Zdroj: | Biomolecules Volume 11 Issue 4 Biomolecules, Vol 11, Iss 592, p 592 (2021) |
| ISSN: | 2218-273X |
| DOI: | 10.3390/biom11040592 |
| Popis: | Spirulina platensis is a “super-food” and has attracted researchers’ attention due toits anti-inflammatory, antioxidant, and analgesic properties. Herein, we investigated the antinociceptive effects of Spirulina in different rodent behavior models of inflammatory pain. Male Swiss mice were treated with Spirulina (3–300 mg/kg, p.o.), indomethacin (10 mg/kg, p.o.), or vehicle (0.9% NaCl 10 mL/kg). Behavioral tests were performed with administration of acetic acid (0.6%, i.p.), formalin 2.7% (formaldehyde 1%, i.pl.), menthol (1.2 µmol/paw, i.pl.), cinnamaldehyde (10 nmol/paw, i.pl.), capsaicin (1.6 µg/paw, i.pl.), glutamate (20 µmol/paw, i.pl.), or naloxone (1 mg/kg, i.p.).The animals were also exposed to the rotarod and open field test to determine possible effects of Spirulina on locomotion and motor coordination. The quantitative phytochemical assays exhibited thatSpirulina contains significant concentrations of total phenols and flavonoid contents, as well as it showed a powerful antioxidant effect with the highest scavenging activity.Oral administration of Spirulina completely inhibited the abdominal contortions induced by acetic acid (ED50 = 20.51 mg/kg). Spirulinatreatment showed significant inhibition of formalin-induced nociceptive behavior during the inflammatory phase, and the opioid-selective antagonist markedly blocked this effect. Furthermore, our data indicate that the mechanisms underlying Spirulina analgesia appear to be related to its ability to modulate TRMP8 and TRPA1, but not by TRPV1 or glutamatergic system. Spirulina represents an orally active and safe natural analgesic that exhibits great therapeutic potential for managing inflammatory pain disorders. |
| Databáze: | OpenAIRE |
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