| Autor: |
Abdurahman, Anwar, Aierken, Wusimanjiang, Zhang, Fei, Obulkasim, Rahmantay, Aniwashi, Jueken, Sulayman, Ablat |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Frontiers in Genetics. 13 |
| ISSN: |
1664-8021 |
| Popis: |
Bone morphogenetic protein receptor type-1B (BMPR1B) is one of the major gene for sheep prolificacy. However, few studies investigated its regulatory region. In this study, we reported that miR-1306 is a direct inhibitor of BMPR1B gene in the ovine granulosa cells (ovine GCs). We detected a miRNA response element of miR-1306 in the 3’ untranslated region of the ovine BMPR1B gene. Luciferase assay showed that the ovine BMPR1B gene is a direct target of miR-1306. qPCR and western blotting revealed that miR-1306 reduces the expression of BMPR1B mRNA and protein in the ovine granulosa cells. Furthermore, miR-1306 promoted cell apoptosis by suppressing BMPR1B expression in the ovine granulosa cells. Overall, our results suggest that miR-1306 is an epigenetic regulator of BMPR1B, and may serve as a potential target to improve the fecundity of sheep. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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