Blood lactate dynamics in awake and anaesthetized mice after intraperitoneal and subcutaneous injections of lactate—sex matters
| Autor: | Øyvind Pernell Haugen, Milada Cvancarova Småstuen, Ingrid Åmellem, Linda H. Bergersen, Jon Storm-Mathisen, Imen Belhaj, Evan Michael Vallenari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Metabolic Sciences Intraperitoneal injections lcsh:Medicine General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Internal medicine Sex differences Blood lactate medicine Intraperitoneal 030304 developmental biology Pharmacology 0303 health sciences Subcutaneous injections Isoflurane business.industry General Neuroscience Subcutaneous Significant difference lcsh:R General Medicine Endocrinology Glucose Lactate General Agricultural and Biological Sciences business 030217 neurology & neurosurgery medicine.drug Neuroscience |
| Zdroj: | PeerJ PeerJ, Vol 8, p e8328 (2020) |
| ISSN: | 2167-8359 |
| Popis: | Lactate treatment has shown a therapeutic potential for several neurological diseases, including Alzheimer’s disease. In order to optimize the administration of lactate for studies in mouse models, we compared blood lactate dynamics after intraperitoneal (IP) and subcutaneous (SC) injections. We used the 5xFAD mouse model for familial Alzheimer’s disease and performed the experiments in both awake and anaesthetized mice. Blood glucose was used as an indication of the hepatic conversion of lactate. In awake mice, both injection routes resulted in high blood lactate levels, mimicking levels reached during high-intensity training. In anaesthetized mice, SC injections resulted in significantly lower lactate levels compared to IP injections. Interestingly, we observed that awake males had significantly higher lactate levels than awake females, while the opposite sex difference was observed during anaesthesia. We did not find any significant difference between transgenic and wild-type mice and therefore believe that our results can be generalized to other mouse models. These results should be considered when planning experiments using lactate treatment in mice. Funding contribution was obtained from the Norwegian Health Association, project ID 14848, project leader Linda H. Bergersen. |
| Databáze: | OpenAIRE |
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