Renoprotective effect of erythropoietin in zebrafish after administration of gentamicin: an immunohistochemical study for β-catenin and c-kit expression

Autor: Carlo Alberto Ricciardi, Michele Buemi, Giuseppe Costantino, Antoine Buemi, Claudia Rifici, Valeria Cernaro, Antonio Lacquaniti, Francesco Macrì, Giulia Maricchiolo, Alessandra Sfacteria, Domenico Santoro
Rok vydání: 2016
Předmět:
Zdroj: JN. Journal of Nephrology (Milano, 1992) 30 (2017): 385–391. doi:10.1007/s40620-016-0353-y
info:cnr-pdr/source/autori:Cernaro V.; Sfacteria A.; Rifici C.; Macri F.; Maricchiolo G.; Lacquaniti A.; Ricciardi C.A.; Buemi A.; Costantino G.; Santoro D.; Buemi M./titolo:Renoprotective effect of erythropoietin in zebrafish after administration of gentamicin: an immunohistochemical study for ?-catenin and c-kit expression/doi:10.1007%2Fs40620-016-0353-y/rivista:JN. Journal of Nephrology (Milano, 1992)/anno:2017/pagina_da:385/pagina_a:391/intervallo_pagine:385–391/volume:30
ISSN: 1724-6059
1121-8428
DOI: 10.1007/s40620-016-0353-y
Popis: Background: Gentamicin is an aminoglycoside antibiotic widely used in the treatment of infections caused by Gram-negative bacteria. The main limitation to its therapeutic effectiveness is the potential nephrotoxicity. Erythropoietin has a tissue protective effect widely demonstrated in the kidney. The aim of the present study was to evaluate the renoprotective effects of erythropoietin in a model of zebrafish (Danio rerio) after administration of gentamicin. Methods: Sixty adult zebrafish were subdivided into three groups: group A was treated with gentamicin; group B received gentamicin and, 24 h later, epoetin alpha; group C received drug diluent only. In order to analyze the renoprotective activity of erythropoietin, the expression of c-kit and ?-catenin was evaluated by immunohistochemistry. Results: Generally, the zebrafish renal tubule regenerates 15 days after an injury. Conversely, 7 days after gentamicin administration, animals treated with erythropoietin (group B) showed a better renal injury repair as documented by: increased expression of ?-catenin, less degenerated tubules, greater number of centers of regeneration, positivity for c-kit only in immature-looking tubules and lymphohematopoietic cells. Conclusion: The expression of c-kit and ?-catenin suggests that erythropoietin may exert a role in regeneration reducing the extent of tubular damage from the outset after gentamicin administration.
Databáze: OpenAIRE
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