Evaluation of C3H/HeJ Mice for Xenodiagnosis of Infection with Ehrlichia Chaffeensis
| Autor: | J M Lockhart, W R Davidson |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences 030106 microbiology Polymerase Chain Reaction Sensitivity and Specificity 0403 veterinary science Mice 03 medical and health sciences medicine Animals Ehrlichia chaffeensis Seroconversion Lymph node DNA Primers Mice Inbred C3H General Veterinary biology Inoculation Deer Ehrlichiosis 04 agricultural and veterinary sciences biology.organism_classification Antibodies Bacterial Virology Titer medicine.anatomical_structure biology.protein Antibody Nested polymerase chain reaction Xenodiagnosis |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 11:55-59 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | Because mice are experimentally susceptible to infection with Ehrlichia species, C3H/HeJmice were evaluated as a potential xenodiagnostic model for detection of infection with and isolation of E. chaffeensis. Intraperitoneal inoculation of mice with E. chaffeensis-infected DH82 cell cultures produced seroconversion, with peak serum antibody titers of 1:256, at high dosages (>1.9 × 104 infected cells) but not at low dosages (1.9 or 1.9 × 102 infected cells). Ehrlichia chaffeensis was not reisolated from blood samples collected from inoculated mice on postinoculation day 21. Nested polymerase chain reaction (PCR), using primers specific for E. chaffeensis, was positive for only 2/70 (2.9%) tissue samples. A field evaluation in which C3H/HeJ mice were inoculated with blood and lymph node suspensions from 5 seropositive white-tailed deer, including 3 deer that were PCR positive for E. chaffeensis, failed to produce seroconversion in mice. The lack of seroconversion at low dosages, the failure to reisolate at any dosage, and the inability to confirm infection in PCR-positive field samples suggests C3H/HeJ mice are not a sensitive model for xenodiagnosis or detection of E. chaffeensis. |
| Databáze: | OpenAIRE |
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