Melatonin Sensitizes OVCAR-3 Cells to Cisplatin through Suppression of PI3K/Akt Pathway

Autor:	Sahar, Baghal-Sadriforoush, Morteza, Bagheri, Isa, Abdi Rad, Fattah, Sotoodehnejadnematalahi
Rok vydání:	2022
Předmět:	Ovarian Neoplasms Phosphatidylinositol 3-Kinases Cell Line Tumor Humans Apoptosis Female General Medicine Cisplatin Reactive Oxygen Species Proto-Oncogene Proteins c-akt Melatonin
Zdroj:	Cellular and Molecular Biology. 68:158-169
ISSN:	1165-158X 0145-5680
DOI:	10.14715/cmb/2022.68.4.19
Popis:	This study examined the effect of melatonin on oxidative stress, expression of pro-apoptotic protein, anti-apoptotic proteins, and the activity of the PI3K/Akt signaling pathway in the human ovarian cancer cell line (OVCAR3). OVCAR3 cells were treated with cisplatin, melatonin, cisplatin + melatonin, and siRNA Akt. Reactive oxygen species levels were assessed. The expression of the proteins was determined by Western blot. Melatonin administration significantly increased intracellular ROS generation, the cleavage of caspase 3 and decreased phosphorylation of Akt. Combination therapy of cisplatin and melatonin increases apoptosis in the OVCAR-3 cells by inhibiting of PI3K/Akt signaling pathway and exacerbating oxidative stress.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::490b095971e0950a089d6395699bf973 https://doi.org/10.14715/cmb/2022.68.4.19 Zobrazit plný text záznamu