Stiffness of Left Ventricular Cardiac Fibroblasts Is Associated With Ventricular Dilation in Patients With Recent-Onset Nonischemic and Nonvalvular Cardiomyopathy
| Autor: | Mathias Busch, Wolfgang Hoffmann, Klaus Empen, Robert Schlicht, Michael Glaubitz, Karin Klingel, Kerstin Weitmann, Stephan Gross, Stephan B. Felix, Marcus Dörr, Christiane A. Helm, Jeannine Witte, Reinhard Kandolf, Stephan Block, Kay E. Gottschalk, Alexander Riad |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Cardiomyopathy Dilated Male medicine.medical_specialty Cardiac fibrosis Heart Ventricles Cardiomyopathy Microscopy Atomic Force Masson's trichrome stain Elastic Modulus Internal medicine Humans Medicine In patient Ventricular dilation Aged business.industry Stiffness General Medicine Fibroblasts Middle Aged medicine.disease Pathophysiology medicine.anatomical_structure Ventricle cardiovascular system Cardiology Female medicine.symptom Cardiology and Cardiovascular Medicine business |
| Zdroj: | Circulation Journal. 78:1693-1700 |
| ISSN: | 1347-4820 1346-9843 |
| DOI: | 10.1253/circj.cj-13-1188 |
| Popis: | BACKGROUND Ventricular dilation is known as a pivotal predictor in recent-onset cardiomyopathy (ROCM), but its pathophysiology is not fully understood. In the present study we investigated whether single-cell stiffness of right and left ventricular-derived fibroblasts has an effect on cardiac phenotype in patients with ROCM. METHODS AND RESULTS Patients with endomyocardial biopsy-proven ROCM were included (n=10). Primary cardiac fibroblasts (CFBs) were cultured from left and right ventricular endomyocardial biopsies and their single-cell stiffness was analyzed by quantification of Young's modulus using colloidal probe atomic force microscopy. Cardiac fibrosis was analyzed by Masson's trichrome staining. CFBs from the left ventricle showed significantly decreased stiffness when compared with CFBs from the right ventricle, indexed by decreased stiffness (Young's modulus 3,374±389 vs. 4,837±690 Pa; P |
| Databáze: | OpenAIRE |
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