The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression
| Autor: | Anton Golovnin, M. M. Erokhin, V. V. Molodina, D. A. Chetverina, P. V. Elizar’ev, M. V. Kostyuchenko, Larisa Melnikova, Pavel Georgiev |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Plasma protein binding DNA-binding protein Article 03 medical and health sciences 0302 clinical medicine Transcription (biology) Animals Drosophila Proteins Protein Interaction Domains and Motifs Binding site Enhancer Promoter Regions Genetic lcsh:Science Psychological repression Zinc finger Multidisciplinary Binding Sites Base Sequence Chemistry lcsh:R Computational Biology Promoter Cell biology DNA-Binding Proteins Repressor Proteins 030104 developmental biology Enhancer Elements Genetic Gene Expression Regulation Drosophila Insulator Elements lcsh:Q 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-41761-6 |
| Popis: | Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in Drosophila. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the specific recognition of the binding site. One of the well-described in vivo function of Su(Hw) is the repression of transcription of neuronal genes in oocytes. Here, we have found that the same Su(Hw) C-terminal region (aa 720–892) is required for insulation as well as for promoter repression. The best characterized partners of Su(Hw), CP190 and Mod(mdg4)-67.2, are not involved in the repression of neuronal genes. Taken together, these results suggest that an unknown protein or protein complex binds to the C-terminal region of Su(Hw) and is responsible for the direct repression activity of Su(Hw). |
| Databáze: | OpenAIRE |
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