Using lysosomal membrane stability of haemocytes in Ruditapes philippinarum as a biomarker of cellular stress to assess contamination by caffeine, ibuprofen, carbamazepine and novobiocin
| Autor: | Ángel DelValls, Sara Buratti, Elena Fabbri, M. Laura Martín-Díaz, G.V. Aguirre-Martínez |
|---|---|
| Přispěvatelé: | Gabriela V. Aguirre-Martínez, Sara Buratti, Elena Fabbri, Angel T. DelVall, M. Laura Martín-Díaz |
| Rok vydání: | 2013 |
| Předmět: |
Neutral red
Environmental Engineering Hemocytes PHARMACEUTICALS HEALTH STATUS HAEMOLYMPH Ruditapes Ibuprofen Pharmacology Biology Wastewater chemistry.chemical_compound MANILA CLAM Caffeine Hemolymph medicine Environmental Chemistry Bioassay Animals Novobiocin General Environmental Science General Medicine Carbamazepine biology.organism_classification BIVALVES Bivalvia bioassay chemistry Neutral Red Environmental chemistry Lysosomes Biomarkers Water Pollutants Chemical medicine.drug |
| Zdroj: | Journal of environmental sciences (China). 25(7) |
| ISSN: | 1001-0742 |
| Popis: | Although pharmaceuticals have been detected in the environment only in the range from ng/L to μg/L, it has been demonstrated that they can adversely affect the health status of aquatic organisms. Lysosomal membrane stability (LMS) has previously been applied as an indicator of cellular well-being to determine health status in bivalve mussels. The objective of this study is to evaluate LMS in Ruditapes philippinarum haemolymph using the neutral red retention assay (NRRA). Clams were exposed in laboratory conditions to caffeine (0.1, 5, 15, 50 μg/L), ibuprofen (0.1, 5, 10, 50 μg/L), carbamazepine and novobiocin (both at 0.1, 1, 10, 50 μg/L) for 35 days. Results show a dose-dependent effect of the pharmaceuticals. The neutral red retention time measured at the end of the bioassay was significantly reduced by 50% after exposure to environmental concentrations (. p < 0.05) (caffeine = 15 μg/L; ibuprofen = 10 μg/L; carbamazepine = 1 μg/L and novobiocin = 1 μg/L), compared to controls. Clams exposed to these pharmaceuticals were considered to present a diminished health status (retention time < 45 min), significantly worse than controls (96 min) (. p < 0.05). The predicted no environmental effect concentration (PNEC) results showed that these pharmaceuticals are very toxic at the environmental concentrations tested. Measurement of the alteration of LMS has been found to be a sensitive technique that enables evaluation of the health status of clams after exposure to pharmaceuticals under laboratory conditions, thus representing a robust Tier-1 screening biomarker. © 2013 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect