Xrn1-resistant RNA structures are well-conserved within the genus flavivirus
| Autor: | Tim J. Dalebout, René C. L. Olsthoorn, Alexander P. Gultyaev, Peter J. Bredenbeek, Assia Bouabda, Ivar W. Dilweg |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
RNA Stability
viruses Genome Viral Virus 03 medical and health sciences Flaviviridae 0302 clinical medicine flavivirus Cricetinae Exoribonuclease RNA pseudoknot Animals Nucleotide sfRNA 3' Untranslated Regions Molecular Biology Xrn1 Cells Cultured Conserved Sequence 030304 developmental biology Subgenomic mRNA chemistry.chemical_classification Genetics 0303 health sciences Base Sequence biology Sequence Analysis RNA virus diseases RNA Cell Biology biochemical phenomena metabolism and nutrition biology.organism_classification exoribonuclease-resistant RNA Flavivirus Culicidae chemistry 030220 oncology & carcinogenesis Exoribonucleases Nucleic Acid Conformation RNA Viral Pseudoknot Research Article Research Paper |
| Zdroj: | RNA Biology, 18(5), 709-717. TAYLOR & FRANCIS INC RNA Biology article-version (VoR) Version of Record RNA Biology, 18(5), 709-717. Informa UK Limited |
| Popis: | Subgenomic RNAs are produced by several RNA viruses through incomplete degradation of their genomic RNA by the exoribonuclease Xrn1, and have been shown to be essential for viral growth and pathogenicity. Within the flavivirus genus of the Flaviviridae family, two distinct classes of Xrn1-resistant RNA motifs have been proposed; one for mosquito-borne and insect-specific flaviviruses, and one for tick-borne flaviviruses and no-known-vector flaviviruses. We investigated tick-borne and no-known-vector flavivirus Xrn1-resistant RNA motifs through systematic in vitro mutational analysis and showed that both classes actually possess very similar structural configurations, including a double pseudoknot and a base-triple at identical, conserved locations. For the no-known-vector flavivirus Modoc virus, we show that in vivo generation of subgenomic flaviviral RNA was affected by mutations targeted at nucleotides involved in the structural features of flaviviral Xrn1-resistant RNA motifs that were defined in this work. Our results suggest that throughout the genus flavivirus Xrn1-resistant RNA motifs adopt the same topologically conserved structure. |
| Databáze: | OpenAIRE |
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