NFκB-Inducing Kinase Deficiency Results in the Development of a Subset of Regulatory T Cells, which Shows a Hyperproliferative Activity upon Glucocorticoid-Induced TNF Receptor Family-Related Gene Stimulation
| Autor: | Zachary A. Scott, Li-Fan Lu, Randolph J. Noelle, David C. Gondek |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Genetic Vectors Immunology Bone Marrow Cells chemical and pharmacologic phenomena Stimulation Receptors Nerve Growth Factor Protein Serine-Threonine Kinases Biology Lymphocyte Activation T-Lymphocytes Regulatory Receptors Tumor Necrosis Factor Cell Line Mice Glucocorticoid-Induced TNFR-Related Protein Transduction Genetic Internal medicine medicine Animals Immunology and Allergy IL-2 receptor Receptor Cells Cultured Cell Line Transformed Cell Proliferation Mice Knockout Kinase Cell growth NF-kappa B Receptors Interleukin-2 hemic and immune systems Dendritic Cells Endocrinology Gene Expression Regulation Cell culture Cancer research Interleukin-2 Glucocorticoid medicine.drug |
| Zdroj: | The Journal of Immunology. 175:1651-1657 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.175.3.1651 |
| Popis: | CD4+CD25+ regulatory T cells (Treg) play an important role in maintaining immunologic tolerance. Glucocorticoid-induced TNFR family-related gene (GITR) expressed preferentially at high levels on Treg has been shown to be a key player of regulating Treg-mediated suppression. A recent study reports that NF-κB-inducing kinase (NIK) expression in thymic stroma is important for the normal production of Treg but not for its suppression capacity. In this report, we have shown that Treg from NIK-deficient mice display hyperproliferative activities upon GITR stimulation through an IL-2-independent mechanism. Furthermore, high dose IL-2, anti-CD28 stimulation, or GITR ligand-transduced bone marrow-derived dendritic cells used as APC (culture conditions which drive Treg proliferation in vitro) could not ablate this difference in proliferative activity between NIK-deficient and wild-type Treg. Additional experiments have shown NIK-deficient mice have a higher ratio of CD4+CD25+CD62Llow Treg both in thymus and periphery than their wild-type littermates. This CD62low subset is responsible for the hyperproliferative activity upon GITR stimulation. These data suggest a novel role of NIK in controlling the development and expansion of CD4+CD25+ regulatory T cells. |
| Databáze: | OpenAIRE |
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