Detection of CD133, Bmi-1, and ABCG2 in ameloblastic tumors

Autor: Kousuke Ohki, Hiroyuki Kumamoto
Rok vydání: 2010
Předmět:
Cancer Research
Pathology
medicine.medical_specialty
Odontogenic Tumors
Biology
medicine.disease_cause
Basement Membrane
Pathology and Forensic Medicine
Ameloblastoma
stomatognathic system
Antigens
CD
Proto-Oncogene Proteins
Carcinoma
medicine
ATP Binding Cassette Transporter
Subfamily G
Member 2
Humans
AC133 Antigen
Glycoproteins
Cell Nucleus
Polycomb Repressive Complex 1
Adamantinoma
Reverse Transcriptase Polymerase Chain Reaction
Enamel Organ
Enamel organ
Endothelial Cells
Nuclear Proteins
Tooth Germ
Odontogenic tumor
Cell Differentiation
Epithelial Cells
Zinc Fingers
Fibroblasts
medicine.disease
Immunohistochemistry
Drug Resistance
Multiple
Neoplasm Proteins
Repressor Proteins
Otorhinolaryngology
embryonic structures
Periodontics
ATP-Binding Cassette Transporters
Stromal Cells
Oral Surgery
Peptides
Carcinogenesis
Clear cell
Zdroj: Journal of Oral Pathology & Medicine. 39:87-93
ISSN: 1600-0714
0904-2512
Popis: J Oral Pathol Med (2010) 39: 87–93 Background: To investigate the roles of stem cell-related molecules in oncogenesis and cytodifferentiation of odontogenic tumors, expression of CD133, Bmi-1, and ATP-binding cassette subfamily G member 2 (ABCG2) was analyzed in ameloblastic tumors as well as in tooth germs. Methods: Tissue specimens of 12 tooth germs, 47 ameloblastomas, and six malignant ameloblastic tumors were examined using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry to determine the expression of CD133, Bmi-1, and ABCG2. Results: mRNA expression of CD133, Bmi-1, and ABCG2 was detected in all samples of normal and neoplastic odontogenic tissues. Immunohistochemical reactivity for CD133 and Bmi-1 was evident in odontogenic epithelial cells neighboring the basement membrane in tooth germs, ameloblastomas, and metastasizing ameloblastomas, and ameloblastic carcinomas and clear cell odontogenic carcinomas showed reactivity for CD133 and Bmi-1 in most neoplastic cells. The level of CD133 immunoreactivity in malignant ameloblastic tumors was significantly higher than the levels in tooth germs and ameloblastomas. Immunoreactivity for ABCG2 in odontogenic epithelial components was detected in some ameloblastic tumors but not in tooth germ tissues. Some granular neoplastic cells in granular cell ameloblastomas showed ABCG2 expression. The level of ABCG2 immunoreactivity in malignant ameloblastic tumors was significantly higher than that in tooth germs. Conclusion: Expression of CD133, Bmi-1, and ABCG2 in tooth germs and ameloblastic tumors suggests that stem cell-related molecules might control the maintenance of odontogenic tissues. Expression of these molecules is possibly involved in oncogenesis, cell differentiation, and malignant potential of odontogenic epithelium.
Databáze: OpenAIRE
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