Electrochemical H2O2 biosensor based on horseradish peroxidase encapsulated protein nanoparticles with reduced graphene oxide-modified gold electrode
| Autor: | Myeong-Jun Lee, Jeong-Hyeop Shin, Byung-Keun Oh, Jin-Ha Choi, Tae Hwan Kim, Ji-ae Song |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Biotechnology
Oxide Nanoparticle 02 engineering and technology macromolecular substances 010402 general chemistry Electrochemistry lcsh:Chemical technology 01 natural sciences Horseradish peroxidase lcsh:Technology law.invention chemistry.chemical_compound law lcsh:TP248.13-248.65 General Materials Science lcsh:TP1-1185 Reduced graphene oxide lcsh:Science biology Full Paper Chemistry Graphene lcsh:T General Engineering technology industry and agriculture Electrochemical 021001 nanoscience & nanotechnology Ascorbic acid Hydrogen peroxide lcsh:QC1-999 0104 chemical sciences Electrode biology.protein lcsh:Q 0210 nano-technology Biosensor lcsh:Physics Nuclear chemistry |
| Zdroj: | Nano Convergence Nano Convergence, Vol 7, Iss 1, Pp 1-8 (2020) |
| ISSN: | 2196-5404 |
| Popis: | In this study, an electrochemical biosensor composed of a horseradish peroxidase (HRP)-encapsulated protein nanoparticles (HEPNP) was fabricated for the sensitive and selective detection of H2O2. The HEPNP has a three-dimensional structure that can contain a large amount of HRP; therefore, HEPNP can amplify the electrochemical signals necessary for the detection of H2O2. Furthermore, reduced graphene oxide (rGO) was used to increase the efficiency of electron transfer from the HEPNP to an electrode, which could enhance the electrochemical signal. This biosensor showed a sensitive electrochemical performance for detection of H2O2 with signals in the range from 0.01–100 μM, and it could detect low concentrations up to 0.01 μM. Furthermore, this biosensor was operated against interferences from glucose, ascorbic acid, and uric acid. In addition, this fabricated H2O2 biosensor showed selective detection performance in human blood serum. Therefore, the proposed biosensor could promote the sensitive and selective detection of H2O2 in clinical applications. |
| Databáze: | OpenAIRE |
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