Low-Fat Diet With Caloric Restriction Reduces White Matter Microglia Activation During Aging

Autor: Zhuoran Yin, Divya D. Raj, Wandert Schaafsma, Roel A. van der Heijden, Susanne M. Kooistra, Aaffien C. Reijne, Xiaoming Zhang, Jill Moser, Nieske Brouwer, Peter Heeringa, Chun-Xia Yi, Gertjan van Dijk, Jon D. Laman, Erik W. G. M. Boddeke, Bart J. L. Eggen
Přispěvatelé: APH - Aging & Later Life, ANS - Cellular & Molecular Mechanisms, Endocrinology Laboratory, Endocrinology, AGEM - Endocrinology, metabolism and nutrition, ACS - Diabetes & metabolism, Van Dijk lab, Critical care, Anesthesiology, Peri-operative and Emergency medicine (CAPE), Groningen Kidney Center (GKC), Translational Immunology Groningen (TRIGR), Molecular Neuroscience and Ageing Research (MOLAR), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
Rok vydání: 2017
Předmět:
0301 basic medicine
medicine.medical_specialty
Saturated fat
Central nervous system
AGED RATS
II COHORT
microglia
Inflammation
C57BL/6J MICE
Biology
Blood–brain barrier
Systemic inflammation
low-fat diet
lcsh:RC321-571
neuroinflammation
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Immune system
LEPTIN RESISTANCE
HYPOTHALAMIC INFLAMMATION
SATURATED FAT
physical exercise
Internal medicine
medicine
Molecular Biology
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Neuroinflammation
Original Research
INSULIN-RESISTANCE
Microglia
BLOOD-BRAIN-BARRIER
aging
INDUCED OBESITY
food and beverages
030104 developmental biology
medicine.anatomical_structure
Endocrinology
high-fat diet
nervous system
LONG-TERM POTENTIATION
caloric restriction
medicine.symptom
030217 neurology & neurosurgery
Neuroscience
Zdroj: Frontiers in Molecular Neuroscience
Frontiers in molecular neuroscience, 11:65. Frontiers Media S.A.
Frontiers in Molecular Neuroscience, Vol 11 (2018)
Frontiers in Molecular Neuroscience, 11:65. Frontiers Media SA
ISSN: 1662-5099
Popis: Rodent models of both aging and obesity are characterized by inflammation in specific brain regions, notably the corpus callosum, fornix, and hypothalamus. Microglia, the resident macrophages of the central nervous system, are important for brain development, neural support, and homeostasis. However, the effects of diet and lifestyle on microglia during aging are only partly understood. Here, we report alterations in microglia phenotype and functions in different brain regions of mice on a high-fat diet (HFD) or low-fat diet (LFD) during aging and in response to voluntary running wheel exercise. We compared the expression levels of genes involved in immune response, phagocytosis, and metabolism in the hypothalamus of 6-month-old HFD and LFD mice. We also compared the immune response of microglia from HFD or LFD mice to peripheral inflammation induced by intraperitoneal injection of lipopolysaccharide (LPS). Finally, we investigated the effect of diet, physical exercise, and caloric restriction (40% reduction compared to ad libitum intake) on microglia in 24-month-old HFD and LFD mice. Changes in diet caused morphological changes in microglia, but did not change the microglia response to LPS-induced systemic inflammation. Expression of phagocytic markers (i.e., Mac-2/Lgals3, Dectin-1/Clec7a, and CD16/CD32) in the white matter microglia of 24-month-old brain was markedly decreased in calorically restricted LFD mice. In conclusion, LFD resulted in reduced activation of microglia, which might be an underlying mechanism for the protective role of caloric restriction during aging-associated decline.
Databáze: OpenAIRE
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