A balancing act in transcription regulation by response regulators: titration of transcription factor activity by decoy DNA binding sites
| Autor: | Zeyue Li, Ti Wu, Samantha E Brokaw, Ann M. Stock, Libby J Helfant, Rong Gao |
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| Rok vydání: | 2021 |
| Předmět: |
Binding Sites
AcademicSubjects/SCI00010 Escherichia coli Proteins Gene regulation Chromatin and Epigenetics Promoter Computational biology DNA Gene Expression Regulation Bacterial Biology DNA binding site Response regulator chemistry.chemical_compound chemistry Genetics Transcriptional regulation Escherichia coli Binding site Nucleotide Motifs Decoy Transcription factor Protein Binding Transcription Factors |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 |
| Popis: | Studies of transcription regulation are often focused on binding of transcription factors (TFs) to a small number of promoters of interest. It is often assumed that TFs are in great excess to their binding sites (TFBSs) and competition for TFs between DNA sites is seldom considered. With increasing evidence that TFBSs are exceedingly abundant for many TFs and significant variations in TF and TFBS numbers occur during growth, the interplay between a TF and all TFBSs should not be ignored. Here, we use additional decoy DNA sites to quantitatively analyze how the relative abundance of a TF to its TFBSs impacts the steady-state level and onset time of gene expression for the auto-activated Escherichia coli PhoB response regulator. We show that increasing numbers of decoy sites progressively delayed transcription activation and lowered promoter activities. Perturbation of transcription regulation by additional TFBSs did not require extreme numbers of decoys, suggesting that PhoB is approximately at capacity for its DNA sites. Addition of decoys also converted a graded response to a bi-modal response. We developed a binding competition model that captures the major features of experimental observations, providing a quantitative framework to assess how variations in TFs and TFBSs influence transcriptional responses. |
| Databáze: | OpenAIRE |
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