An E. coli expression system optimized for DELLA proteins
| Autor: | Toshi Foster, Xiaolin Sun, Taha H. Al-Samarrai, William T. Jones, Jasna Rakonjac, Dawn Harvey, Christopher A. Kirk, Nicky Frearson |
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| Rok vydání: | 2008 |
| Předmět: |
Malus
Plant growth medicine.drug_class Recombinant Fusion Proteins Genetic Vectors Arabidopsis Genes Plant medicine.disease_cause Monoclonal antibody Gene Expression Regulation Plant Auxin Escherichia coli medicine Cloning Molecular Plant Proteins chemistry.chemical_classification biology fungi food and beverages biology.organism_classification Plant biology Molecular biology Gibberellins Cell biology chemistry Plasmids Biotechnology |
| Zdroj: | Protein Expression and Purification. 58:168-174 |
| ISSN: | 1046-5928 |
| DOI: | 10.1016/j.pep.2007.09.003 |
| Popis: | The DELLA proteins are involved in regulation of plant growth in response to phytohormonal signals such as GA, ethylene, and auxin. They have become one of most challenging and active area of research due to their fundamental roles in plant biology. Here, we describe the first successful expression of the N-terminal domains of DELLA proteins of Arabidopsis thaliana and Malus domestica in Escherichia coli system which will be used to produce monoclonal antibodies for profiling protein micro-arrays. Optimizations of the cloning, expression, and purification using specific tags have been discussed. |
| Databáze: | OpenAIRE |
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