β-Bungarotoxin inhibition of calcium accumulation by rat brain mitochondria
Autor: | Regis B. Kelly, Gail M. Wagner, Patricia E. Mart |
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Rok vydání: | 1974 |
Předmět: |
Time Factors
Biophysics chemistry.chemical_element Stimulation Mitochondrion Biology Calcium medicine.disease_cause Biochemistry chemistry.chemical_compound Centrifugation Density Gradient medicine Animals Mitochondrial calcium uptake Neurotransmitter Molecular Biology Toxin Calcium Radioisotopes Temperature Brain Biological Transport Succinates Cell Biology Bungarotoxin Bungarotoxins Mitochondria Kinetics Electrophysiology chemistry Dinitrophenols |
Zdroj: | Biochemical and Biophysical Research Communications. 58:475-481 |
ISSN: | 0006-291X |
DOI: | 10.1016/0006-291x(74)90389-1 |
Popis: | Electrophysiological studies have shown that β-bungarotoxin modifies the release of neurotransmitter from mammal ian motor-nerve terminals. In this paper we demonstrate that β-bungarotoxin can also inhibit calcium accumulation into sub-cellular fractions from rat brain at very low concentrations (2–8 pmoles toxin/mg protein). Since the calcium uptake which is inhibited has the characteristics of mitochondrial calcium accumulation (DNP-sensitivity, succinate stimulation), we conclude that the toxin affects the mitochondria. We suggest that the electrophysiological observations are consistent with direct or indirect inhibition by toxin of mitochondrial calcium uptake. |
Databáze: | OpenAIRE |
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