Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L
| Autor: | Qian, Liao, Lan, Pang, Jing-Jing, Li, Chen, Zhang, Jia-Xing, Li, Xing, Zhang, Ting, Mao, Ding-Tao, Wu, Xiu-Ying, Ma, Fu-Neng, Geng, Jin-Ming, Zhang |
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| Rok vydání: | 2022 |
| Předmět: |
Wound Healing
Chemical Phenomena Macromolecular Substances Spectrum Analysis Monosaccharides Biocompatible Materials General Medicine Biochemistry Cell Line Diabetes Mellitus Experimental Mice Polysaccharides Structural Biology Animals Humans Insect Proteins Periplaneta Medicine Traditional Amino Acids Molecular Biology |
| Zdroj: | International Journal of Biological Macromolecules. 195:466-474 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2021.12.018 |
| Popis: | Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings. In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession. Among these isolated fractions, two fractions eluted by 0.3 M and 0.5 M NaCl with the higher yield, respectively named PaPPc2 and PaPPc3 respectively, were chosen for the wound healing experiments. Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and |
| Databáze: | OpenAIRE |
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