Whole genome analysis of a Vietnamese trio
| Autor: | Nguyen Dai Thanh, Pham Bao Son, Le Si Quang, Nguyen Huu Duc, Phan Thi Thu Hang, Do Duc Dong, Le Sy Vinh, Bui Quang Minh, Dang Cao Cuong, Pham Thi Minh Trang, Dang Thanh Hai, Hoang Kim Phuc |
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| Rok vydání: | 2015 |
| Předmět: |
Genetics
Base Sequence Contig Genome Human Sequence assembly Single-nucleotide polymorphism DNA Sequence Analysis DNA General Medicine Biology Polymorphism Single Nucleotide Genome General Biochemistry Genetics and Molecular Biology Human genetics Asian People INDEL Mutation Vietnam Ethnicity Humans Family Human genome General Agricultural and Biological Sciences Indel Reference genome |
| Zdroj: | Journal of Biosciences. 40:113-124 |
| ISSN: | 0973-7138 0250-5991 |
| DOI: | 10.1007/s12038-015-9501-0 |
| Popis: | We here present the first whole genome analysis of an anonymous Kinh Vietnamese (KHV) trio whose genomes were deeply sequenced to 30-fold average coverage. The resulting short reads covered 99.91 percent of the human reference genome (GRCh37d5). We identified 4,719,412 SNPs and 827,385 short indels that satisfied the Mendelian inheritance law. Among them, 109,914 (2.3 percent) SNPs and 59,119 (7.1 percent) short indels were novel. We also detected 30,171 structural variants of which 27,604 (91.5 percent) were large indels. There were 6,681 large indels in the range 0.1-100 kbp occurring in the child genome that were also confirmed in either the father or mother genome. We compared these large indels against the DGV database and found that 1,499 (22.44 percent) were KHV specific. De novo assembly of high-quality unmapped reads yielded 789 contigs with the length greater than or equal to 300 bp. There were 235 contigs from the child genome of which 199 (84.7 percent) were significantly matched with at least one contig from the father or mother genome. Blasting these 199 contigs against other alternative human genomes revealed 4 novel contigs. The novel variants identified from our study demonstrated the necessity of conducting more genome-wide studies not only for Kinh but also for other ethnic groups in Vietnam. |
| Databáze: | OpenAIRE |
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