Fibrinogen is localized on dark zone follicular dendritic cells in vivo and enhances the proliferation and survival of a centroblastic cell line in vitro
| Autor: | L. S. Brackenbury, Lawrence G. Hunt, Paul Monaghan, Eric A. Lefevre, Emma A. Supple, Zania Stamataki, Yolande Richard, Wayne R. Hein, Bryan Charleston, Gwenoline Borhis |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Carcinoma
Hepatocellular Cell Survival medicine.drug_class T-Lymphocytes Molecular Sequence Data Palatine Tonsil Immunology Lymphocyte Activation Monoclonal antibody Affinity maturation Mice Tumor Cells Cultured medicine Animals Humans Immunology and Allergy Amino Acid Sequence B cell Cell Proliferation Mice Inbred BALB C Sheep Sequence Homology Amino Acid biology Follicular dendritic cells Liver Neoplasms Antibodies Monoclonal Fibrinogen Germinal center Cell Differentiation Receptors Antigen T-Cell gamma-delta Cell Biology Germinal Center Burkitt Lymphoma Molecular biology In vitro medicine.anatomical_structure Cell culture biology.protein Cattle Immunization Antibody Dendritic Cells Follicular Signal Transduction |
| Zdroj: | Journal of Leukocyte Biology. 82:666-677 |
| ISSN: | 1938-3673 0741-5400 |
| Popis: | Follicular dendritic cells (FDC) in the germinal centers (GC) of secondary lymphoid or- gans increase the survival and proliferation of an- tigen-stimulated B cells and are pivotal for the affinity maturation of an antibody response and for maintenance of B cell immunological memory. The dark zone (DZ) and the light zone (LZ) constitute distinct areas of the GC containing different sub- types of FDC as identified by their morphology and phenotype. Until now, most available FDC-specific reagents identify LZ FDC, and there are no re- agents recognizing DZ FDC specifically. Here, we report a new mAb, D46, which stains FDC specif- ically in the DZ of bovine and ovine GC within the secondary follicles. We identify its ligand as bovine fibrinogen, and using commercially available anti- human fibrinogen antibodies, show that this inflam- matory protein is also present on DZ FDC of hu- man GC within palatine tonsils. In vitro, the addi- tion of exogenous fibrinogen stimulates the proliferation and survival of BCR-stimulated L3055 cells, which constitute a clonal population of centroblastic cells and retain important features of normal GC B cells. Together, our results suggest that fibrinogen localized on DZ FDC could support the extensive proliferation and survival of GC B cells within the DZ in vivo. J. Leukoc. Biol. 82: 666-677; 2007. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text