Extracellular β-1,3-glucanases are induced during early somatic embryogenesis in Cichorium
| Autor: | Jean-Louis Hilbert, Guy Bauw, Lionel Belingheri, Stéphane Helleboid, Jacques Vasseur |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Somatic embryogenesis
Molecular Sequence Data Plant Science Biology Chicory chemistry.chemical_compound Cichorium Genetics Extracellular Amino Acid Sequence Peptide sequence Plant Proteins chemistry.chemical_classification Sequence Homology Amino Acid beta-Glucosidase Callose Glucan 1 3-beta-Glucosidase Glucanase Plants Genetically Modified biology.organism_classification chemistry Biochemistry Culture Media Conditioned Enzyme Induction Seeds Chitinase biology.protein Extracellular Space Glycoprotein |
| Zdroj: | Planta. 205:56-63 |
| ISSN: | 1432-2048 0032-0935 |
| Popis: | In leaf tissues of the Cichorium hybrid clone '474' (C. intybus L. var. sativum x C. endivia L. var. latifolia), the acquisition and expression of embryogenic competence was characterised by the appearance of 15 polypeptides (Boyer et al., 1993, Plant Sci 93: 41-53). The 38-kDa proteins were found to be abundantly present in conditioned embryogenic medium after the first division of the induced cells. These proteins seemed to be glycosylated as indicated by general carbohydrate detection methods. Internal amino-acid sequences obtained after microsequencing tryptic peptides appeared to be 36-57% homologous with plant beta-1,3-endoglucanases. In addition, these 38-kDa proteins were recognised by antibodies raised against the pathogenesis-related tobacco glucanase PR2a and their beta-1,3-glucanase activity was demonstrated by direct detection in polyacrylamide gels after electrophoresis. These results strongly suggested that the 38-kDa somatic-embryogenesis-related (SER) polypeptides are beta-1,3-glucanases. Moreover, the level of glucanase activity was nearly three times higher in the medium of the embryogenic '474' line than in the medium of a non-embryogenic line. The possible involvement of the extracellular 38-kDa proteins in callose degradation during somatic embryogenesis is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink