Recombinant Human Melatonin Receptor MT1 Isolated in Mixed Detergents Shows Pharmacology Similar to That in Mammalian Cell Membranes
| Autor: | Logez, C. (Christel), Berger, S. (Sylvie), Legros, C. (Céline), Banères, J. (Jean-Louis), Cohen, W. (William), Delagrange, P. (Philippe), Nosjean, O. (Olivier), Boutin, Jean A., Ferry, G. (Gilles), Simonin, F. (Frederic), Wagner, R. (Renaud), Tosini, G. (Gianluca) (editor) |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Protein Structure
Science Detergents Cell Membranes Gene Expression Plasma protein binding macromolecular substances CHO Cells Ligands Melatonin receptor Biochemistry Protein Chemistry Pichia pastoris law.invention Cell Line Cell membrane Cricetulus law Yeasts medicine Escherichia coli Animals Humans Receptor G protein-coupled receptor Multidisciplinary biology Chinese hamster ovary cell Receptor Melatonin MT1 Cell Membrane Biology and Life Sciences Proteins Membrane Proteins Sciences du Vivant [q-bio]/Biotechnologies Cell Biology biology.organism_classification Recombinant Proteins medicine.anatomical_structure Recombinant DNA Cytochemistry Medicine Cellular Structures and Organelles Protein Binding Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 6, p e100616 (2014) |
| Popis: | The human melatonin MT1 receptor—belonging to the large family of G protein-coupled receptors (GPCRs)—plays a key role in circadian rhythm regulation and is notably involved in sleep disorders and depression. Structural and functional information at the molecular level are highly desired for fine characterization of this receptor; however, adequate techniques for isolating soluble MT1 material suitable for biochemical and biophysical studies remain lacking. Here we describe the evaluation of a panel of constructs and host systems for the production of recombinant human MT1 receptors, and the screening of different conditions for their solubilization and purification. Our findings resulted in the establishment of an original strategy using a mixture of Fos14 and CHAPS detergents to extract and purify a recombinant human MT1 from Pichia pastoris membranes. This procedure enabled the recovery of relatively pure, monomeric and ligand-binding active MT1 receptor in the near-milligram range. A comparative study based on extensive ligand-binding characterization highlighted a very close correlation between the pharmacological profiles of MT1 purified from yeast and the same receptor present in mammalian cell membranes. The high quality of the purified MT1 was further confirmed by its ability to activate its cognate Gαi protein partner when reconstituted in lipid discs, thus opening novel paths to investigate this receptor by biochemical and biophysical approaches. |
| Databáze: | OpenAIRE |
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