Transfer RNA modifications that alter +1 frameshifting in general fail to affect −1 frameshifting
| Autor: | Samia Sn Ben Salem, Jérôme M. B. Durand, Philip J. Farabaugh, Guillaume Stahl, Jaunius Urbonavičius, Qiang Q Qian, Glenn R. Björk |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Salmonella typhimurium
TRNA modification Saccharomyces cerevisiae Mutant Congenic Biology medicine.disease_cause Article Frameshift mutation RNA Transfer medicine Anticodon Escherichia coli Molecular Biology Gene Genetics Base Sequence Models Genetic Frameshifting Ribosomal Nucleosides biology.organism_classification Biochemistry Transfer RNA Mutation |
| Popis: | Using mutants (tgt, mnmA(asuE, trmU), mnmE(trmE), miaA, miaB, miaE, truA(hisT), truB) of either Escherichia coli or Salmonella enterica serovar Typhimurium and the trm5 mutant of Saccharomyces cerevisiae, we have analyzed the influence by the modified nucleosides Q34, mnm5s2U34, ms2io6A37, Ψ39, Ψ55, m1G37, and yW37 on −1 frameshifts errors at various heptameric sequences, at which at least one codon is decoded by tRNAs having these modified nucleosides. The frequency of −1 frameshifting was the same in congenic strains only differing in the allelic state of the various tRNA modification genes. In fact, in one case (deficiency of mnm5s2U34), we observed a reduced ability of the undermodified tRNA to make a −1 frameshift error. These results are in sharp contrast to earlier observations that tRNA modification prevents +1 frameshifting suggesting that the mechanisms by which −1 and +1 frameshift errors occur are different. Possible mechanisms explaining these results are discussed. |
| Databáze: | OpenAIRE |
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