Acetylcholinesterase plays a non-neuronal, non-esterase role in organogenesis
| Autor: | Michael K. Dush, Nanette Nascone-Yoder, Melissa A. Pickett |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Research Report
0301 basic medicine Nervous system Embryo Nonmammalian Morpholino Organogenesis Population Morphogenesis Xenopus Biology Nervous System Xenopus laevis 03 medical and health sciences chemistry.chemical_compound Cell Adhesion medicine Animals RNA Messenger education Molecular Biology education.field_of_study Endoderm Cell Biology biology.organism_classification Immunohistochemistry Acetylcholinesterase Acetylcholine Fibronectins Cell biology 030104 developmental biology medicine.anatomical_structure Biochemistry chemistry Cholinergic Developmental Biology medicine.drug |
| Zdroj: | Journal of Cell Science. 130:e1.1-e1.1 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.209031 |
| Popis: | Acetylcholinesterase (AChE) is crucial for degrading acetylcholine at cholinergic synapses. In vitro studies suggest that, in addition to its role in nervous signaling, AChE can also modulate non-neuronal cell properties, although it remains controversial whether AChE functions in this capacity in vivo. Here, we show that AChE plays an essential non-classical role in vertebrate gut morphogenesis. Exposure of Xenopus embryos to AChE-inhibiting chemicals results in severe defects in intestinal development. Tissue-targeted loss of function assays (via microinjection of antisense morpholino or CRISPR-Cas9) confirm that AChE is specifically required in the gut endoderm tissue, a non-neuronal cell population, where it mediates adhesion to fibronectin and regulates cell rearrangement events that drive gut lengthening and digestive epithelial morphogenesis. Notably, the classical esterase activity of AChE is dispensable for this activity. As AChE is deeply conserved, widely expressed outside of the nervous system, and the target of many environmental chemicals, these results have broad-reaching implications for development and toxicology. |
| Databáze: | OpenAIRE |
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