Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury

Autor: Lee G. Fradkin, Matthew R. J. Mason, Martijn J A Malessy, Martijn R. Tannemaat, Joost Verhaagen, Marlijn van der Poel, Jinhui Lee, Arie C van Vliet, Fred de Winter, Jasprina N Noordermeer
Přispěvatelé: Molecular and Cellular Neurobiology, Amsterdam Neuroscience - Neurodegeneration, Netherlands Institute for Neuroscience (NIN), Graduate School
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Macroglial Cells
Gene Expression
Nervous System
Nerve Fibers
Cell Signaling
Peripheral Nerve Injuries
Animal Cells
Ganglia
Spinal
Medicine and Health Sciences
Morphogenesis
Axon
Receptor
Wnt Signaling Pathway
WNT Signaling Cascade
Neurons
Multidisciplinary
Nerves
Wnt signaling pathway
Sciatic Nerve
Signaling Cascades
Cell biology
WNT5A
medicine.anatomical_structure
Medicine
Female
Sciatic nerve
Cellular Types
Anatomy
medicine.symptom
Research Article
Signal Transduction
Sensory Receptor Cells
Science
Glial Cells
Biology
Sciatic Nerves
SDG 3 - Good Health and Well-being
Downregulation and upregulation
Genetics
medicine
Animals
Humans
Regeneration
Gene Regulation
Rats
Wistar
Regeneration (biology)
Biology and Life Sciences
Cell Biology
Nerve injury
Axons
Nerve Regeneration
Rats
Disease Models
Animal
Gene Expression Regulation
Cellular Neuroscience
Schwann Cells
Organism Development
Neuroscience
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, 16(4). PUBLIC LIBRARY SCIENCE
PLoS ONE, Vol 16, Iss 4, p e0249748 (2021)
PLoS ONE, 16(4 April):e0249748. Public Library of Science
PLoS One, 16(4). Public Library of Science
Van Vliet, A C, Lee, J, Van Der Poel, M, Mason, M R J, Noordermeer, J N, Fradkin, L G, Tannemaat, M R, Malessy, M J A, Verhaagen, J & De Winter, F 2021, ' Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury ', PLoS ONE, vol. 16, no. 4 April, e0249748 . https://doi.org/10.1371/journal.pone.0249748
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0249748
Popis: A human neuroma-in continuity (NIC), formed following a peripheral nerve lesion, impedes functional recovery. The molecular mechanisms that underlie the formation of a NIC are poorly understood. Here we show that the expression of multiple genes of the Wnt family, including Wnt5a, is changed in NIC tissue from patients that underwent reconstructive surgery. The role of Wnt ligands in NIC pathology and nerve regeneration is of interest because Wnt ligands are implicated in tissue regeneration, fibrosis, axon repulsion and guidance. The observations in NIC prompted us to investigate the expression of Wnt ligands in the injured rat sciatic nerve and in the dorsal root ganglia (DRG). In the injured nerve, four gene clusters were identified with temporal expression profiles corresponding to particular phases of the regeneration process. In the DRG up- and down regulation of certain Wnt receptors suggests that nerve injury has an impact on the responsiveness of injured sensory neurons to Wnt ligands in the nerve. Immunohistochemistry showed that Schwann cells in the NIC and in the injured nerve are the source of Wnt5a, whereas the Wnt5a receptor Ryk is expressed by axons traversing the NIC. Taken together, these observations suggest a central role for Wnt signalling in peripheral nerve regeneration.
Databáze: OpenAIRE
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