Characterization of expressed human meibum using hyperspectral stimulated Raman scattering microscopy
Autor: | Jeremiah P. Tao, Sumit Garg, Marjan Farid, Jerry R. Paugh, Eric O. Potma, Andrew Loc Nguyen, Donald J. Brown, Alba Alfonso-Garcia, James V. Jester, Jeffrey L. Suhalim |
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Rok vydání: | 2018 |
Předmět: |
Adult
Male Adolescent Nonlinear Optical Microscopy Dry eye Ophthalmology & Optometry Human meibum Article Machine Learning 03 medical and health sciences Young Adult 0302 clinical medicine Nuclear magnetic resonance Opthalmology and Optometry Microscopy 80 and over Humans Inverse correlation 030304 developmental biology Aged Aged 80 and over 0303 health sciences Stimulated Raman Scattering Microscopy Chemistry High protein Meibomian gland dysfunction Meibomian Glands Reproducibility of Results Protein composition Middle Aged Lipid Metabolism Vertex component analysis Ophthalmology Tears Raman spectroscopy 030221 ophthalmology & optometry Eyelid Diseases Female sense organs |
Zdroj: | The ocular surface, vol 17, iss 1 |
ISSN: | 1937-5913 |
Popis: | Purpose This study examined whether hyperspectral stimulated Raman scattering (hsSRS) microscopy can detect differences in meibum lipid to protein composition of normal and evaporative dry eye subjects with meibomian gland dysfunction. Methods Subjects were evaluated for tear breakup time (TBUT), staining, meibum expression and gland dropout. Expressed meibum was analyzed using SRS vibrational signatures in the CH stretching region (2800–3050 cm−1). Vertex component analysis and K-means clustering were used to group the spectral signatures into four fractions containing high lipid (G1) to high protein (G4). Results Thirty-three subjects could be statistically analyzed using pooled meibum (13 with stable tear films (TBUTs > 10 s) and 20 with unstable tear films (TBUTs ≤ 10 s). Significant differences in meibum from subjects with unstable vs. stable TBUTs were found for the G1 fraction (medians 0.164 and 0.020, respectively; p = 0.012) and the G2 fraction (medians 0.244 and 0.272, respectively; p = 0.045). No differences were observed for the G3 and G4 fractions. Single orifice samples were not significantly different vs. pooled samples from the fellow eye, and eyelid sector samples (nasal, central and temporal) G2:G3 fractional components were not significantly different (p = 0.449). Spearman analysis suggested a significant inverse correlation between G1 fraction and TBUT (R = −0.351; p = 0.045). Conclusions hsSRS microscopy allows compositional analysis of expressed meibum from humans which correlated to changes in TBUT. These findings support the hypothesis that hsSRS may be useful in classifying meibum quality and evaluating the effects of therapy. |
Databáze: | OpenAIRE |
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