Identification of the early VIP-regulated transcriptome and its associated, interactome in resting and activated murine CD4 T cells
| Autor: | Erich Raymond Wilkerson, Travis Van der Steen, Yulia Dementieva, James Denvir, Goran Boskovic, Glenn Dorsam, Emilie E. Vomhof-DeKrey, Rebecca J Hermann, Sheri T. Dorsam, Jodie S. Haring, Donald A. Primerano |
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| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Regulatory T cell T cell Immunology Vasoactive intestinal peptide Biology Lymphocyte Activation Article Transcriptome Mice chemistry.chemical_compound Immune system medicine Animals Gene Regulatory Networks IL-2 receptor Molecular Biology Oligonucleotide Array Sequence Analysis Regulation of gene expression Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Reproducibility of Results Molecular biology Mice Inbred C57BL Phenotype medicine.anatomical_structure Gene Expression Regulation chemistry Ionomycin Receptors Vasoactive Intestinal Peptide Tetradecanoylphorbol Acetate Female hormones hormone substitutes and hormone antagonists Signal Transduction Vasoactive Intestinal Peptide |
| Zdroj: | Molecular Immunology. 47:1181-1194 |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/j.molimm.2010.01.003 |
| Popis: | More than 40 years after the discovery of vasoactive intestinal peptide (VIP), its transcriptome in the immune system has still not been completely elucidated. In an attempt to understand the biological role of this neuropeptide in immunity, we chose CD4 T cells as a cellular system. Agilent Mouse Whole Genome microarrays were hybridized with fluorescently labeled total RNA isolated from resting CD4 T cells cultured −/+ 10−7 M VIP for five hours or PMA/ionomycin activated CD4 T cells cultured −/+ 10−7 M VIP for five hours . These VIP-regulated transcriptomes were analyzed by Significance Analysis of Microarrays (SAM) and Ingenuity Pathway Analysis (IPA) software to identify relevant signaling pathways modulated by VIP in the absence and presence of T cell activation. In resting CD4 T cells, VIP modulated 368 genes, ranging from 3.49 to − 4.78 fold. In the PMA/ionomycin activated CD4 T cells, 326 gene expression levels were changed by VIP, ranging from 2.94 to −1.66 fold. IPA analysis revealed that VIP exposure alters cellular function through EGFR signaling in resting CD4 T cells, and modulates immediate early genes, Fos and CREM/ICER, in activated CD4 T cells. These gene expression changes are suggested to explain at a molecular level how VIP can regulate T cell homing to the gut and induce regulatory T cell generation. |
| Databáze: | OpenAIRE |
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