An Ionic Liquid Extraction That Preserves the Molecular Structure of Cutin Shown by Nuclear Magnetic Resonance
| Autor: | Christophe Rothan, Ângela Pinheiro, Łukasz P. Haliński, Rita Escórcio, Johann Petit, Carlos J. S. Moreira, Vanessa G. Correia, Cristina Silva Pereira, Joana Pais, Artur Bento, Oleksandr O. Mykhaylyk |
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| Přispěvatelé: | Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Gdańsk (UG), Department of Chemistry [Sheffield], University of Sheffield [Sheffield], Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Bordeaux (UB) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Magnetic Resonance Spectroscopy Physiology Cuticle [SDV]Life Sciences [q-bio] Ionic Liquids Plante fruitière Plant Science Cutin engineering.material Polysaccharide 01 natural sciences chemistry.chemical_compound Membrane Lipids Solanum lycopersicum Fruit charnu Cutine Tomate Genetics Organic chemistry Molecule [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Polyester végétal Research Articles chemistry.chemical_classification fungi Imidazoles food and beverages Polyester Monomer chemistry Ionic liquid engineering Microscopy Electron Scanning Biopolymer Cuticule 010606 plant biology & botany |
| Zdroj: | Plant Physiol Plant Physiology Plant Physiology, American Society of Plant Biologists, 2020, 184 (2), pp.592-606. ⟨10.1104/pp.20.01049⟩ |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.20.01049⟩ |
| Popis: | The biopolyester cutin is ubiquitous in land plants, building the polymeric matrix of the plant's outermost defensive barrier, the cuticle. Cutin influences many biological processes in planta; however, due to its complexity and highly branched nature, the native structure remains partially unresolved. Our aim was to define an original workflow for the purification and systematic characterization of the molecular structure of cutin. To purify cutin we tested the ionic liquids cholinium hexanoate and 1-butyl-3-methyl-imidazolium acetate. The ensuing polymeric materials are highly esterified, amorphous, and have a typical monomeric composition as demonstrated by solid-state NMR, complemented by spectroscopic, thermal, and x-ray scattering analyses. We performed a systematic study by solution-state NMR of cryogenically milled cutins extracted from tomatoes (Solanum lycopersicum 'Micro-Tom'; the wild type and the GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE [GPAT6] and CUTIN SYNTHASE [CUS1] mutants). We resolved their molecular structures, relative distribution of ester aliphatics, free acid end-groups and free hydroxyl groups, differentiating between those derived from primary and secondary esters. Our data demonstrate the existence of free hydroxyl groups in cutin and provide insight into how the mutations affect the esterification arrangement of cutin. The usage of ionic liquids for studying plant polyesters has advantages over conventional approaches, since simple modifications can be applied to recover a biopolymer carrying distinct types/degrees of modifications (e.g. preservation of esters or cuticular polysaccharides), which in combination with the solution NMR methodologies developed here, constitutes essential tools to fingerprint the multifunctionality and the structure of cutin in planta. |
| Databáze: | OpenAIRE |
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