Dissociation of collagenase-tissue inhibitor of metalloproteinases-1 (TIMP-1) complex - its application for the independent measurements of TIMP-1 and collagenase activity in crude culture media and body fluids
| Autor: | Liming Zou, Hirohisa Hayakawa, Jian Zhang, Ikuyo Kondo, Kazushi Iwata, Noboru Fujimoto, Taro Hayakawa, Osamu Narita, Kyoko Yamashita, Toshihide Noguchi |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Matrix metalloproteinase inhibitor Gingiva Human skin Matrix Metalloproteinase Inhibitors Matrix metalloproteinase Chromatography Affinity Rheumatology medicine Humans Collagenases Fibroblast Lung Cells Cultured Glycoproteins chemistry.chemical_classification biology Tissue Inhibitor of Metalloproteinases Fibroblasts Body Fluids Culture Media medicine.anatomical_structure Enzyme Biochemistry chemistry Enzyme inhibitor biology.protein Collagenase Interstitial collagenase Female medicine.drug |
| Zdroj: | Matrix. 12:481-487 |
| ISSN: | 0934-8832 |
| Popis: | Collagenase-tissue inhibitor of metalloproteinases-1 (TIMP-1) complex was prepared from activated collagenase and TIMP-1 purified from culture media of human skin fibroblasts. After having been confirmed to be a complex by zinc chelate chromatography, the complex was demonstrated to dissociate by passage through an anti-TIMP-1 monoclonal antibody-affinity column. On the basis of above evidence, a simple strategy was set up for the independent measurements of TIMP-1 concentration, and both active and total collagenase activities in crude culture media and body fluids. |
| Databáze: | OpenAIRE |
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