Oxidative degradation of model lipids representative for main paper pulp lipophilic extractives by the laccase-mediator system
| Autor: | Ana Gutiérrez, Ángel T. Martínez, José C. del Río, Jorge Rencoret, Eric Record, Setefilla Molina, Anne Lomascolo |
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| Přispěvatelé: | Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Unité mixte de recherche de biotechnologie des champignons filamenteux, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Université de Provence - Aix-Marseille 1, Centro de Investigaciones Biologicas |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
01 natural sciences Applied Microbiology and Biotechnology Lipid peroxidation chemistry.chemical_compound PAPER PULP Organic chemistry chemistry.chemical_classification Eucalyptus 0303 health sciences biology General Medicine FUNGAL ENZYME Lipids paper pulps REACTION MECHANISMS [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Saturated fatty acid GAS CHROMATOGRAPHY lipids (amino acids peptides and proteins) OXIDATIVE ENZYME Oxidation-Reduction Biotechnology Polyunsaturated fatty acid Paper Chromatography Gas MODEL LIPIDS Fungal Proteins Industrial Microbiology 03 medical and health sciences 010608 biotechnology pitch deposits Unsaturated fatty acid 030304 developmental biology Laccase-mediator system Chromatography PITCH DEPOSITS Laccase Fatty acid PYCNOPORUS CINNABARINUS Pycnoporus cinnabarinus LACCASE-MEDIATOR SYSTEM Triazoles Lipid Metabolism biology.organism_classification Sterol reaction mechanisms fungal enzymes chemistry model lipids Lipid modification Polyporales |
| Zdroj: | Applied Microbiology and Biotechnology Applied Microbiology and Biotechnology, Springer Verlag, 2008, 80 (2), pp.211-222. ⟨10.1007/s00253-008-1547-6⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0175-7598 1432-0614 |
| DOI: | 10.1007/s00253-008-1547-6⟩ |
| Popis: | E-mail: anagu@irnase.csic.es (A. Gutiérrez) Different model lipids—alkanes, fatty alcohols, fatty acids, resin acids, free sterols, sterol esters, and triglycerides—were treated with Pycnoporus cinnabarinus laccase in the presence of 1-hydroxybenzotriazole as mediator, and the products were analyzed by gas chromatography. The laccase alone decreased the concentration of some unsaturated lipids. However, the most extensive lipid modification was obtained with the laccase–mediator system. Unsaturated lipids were largely oxidized and the dominant products detected were epoxy and hydroxy fatty acids from fatty acids and free and esterified 7-ketosterols and steroid ketones from sterols and sterol esters. The former compounds suggested unsaturated lipid attack via the corresponding hydroperoxides. The enzymatic reaction on sterol esters largely depended on the nature of the fatty acyl moiety, i.e., oxidation of saturated fatty acid esters started at the sterol moiety, whereas the initial attack of unsaturated fatty acid esters was produced on the fatty acid double bonds. In contrast, saturated lipids were not modified, although some of them decreased when the laccase–mediator reactions were carried out in the presence of unsaturated lipids suggesting participation of lipid peroxidation radicals. These results are discussed in the context of enzymatic control of pitch to explain the removal of lipid mixtures during laccase–mediator treatment of different pulp types. |
| Databáze: | OpenAIRE |
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