Gene expression studies using a miniaturized thermal cycler system on board the International Space Station

Autor: Sebastian Kraves, Ezequiel Alvarez Saavedra, D. Scott Copeland, Tessa G. Montague, Emily J. Gleason, Alia Almansoori, Kevin Foley
Rok vydání: 2018
Předmět:
0301 basic medicine
Science and Technology Workforce
Nematoda
Computer science
lcsh:Medicine
Gene Expression
Artificial Gene Amplification and Extension
Careers in Research
Polymerase Chain Reaction
law.invention
0302 clinical medicine
law
lcsh:Science
DNA extraction
Electrophoresis
Agar Gel
Multidisciplinary
Thermal cycler
Reverse Transcriptase Polymerase Chain Reaction
Human spaceflight
Eukaryota
Mars Exploration Program
Animal Models
DNA
Helminth
Professions
Experimental Organism Systems
030220 oncology & carcinogenesis
Astronauts
Research Article
Science Policy
Computational biology
Spaceflight
Research and Analysis Methods
03 medical and health sciences
Extraction techniques
Model Organisms
International Space Station
Genetics
Animals
Humans
Caenorhabditis elegans
Molecular Biology Techniques
Molecular Biology
Weightlessness
lcsh:R
Organisms
Biology and Life Sciences
Reverse Transcription
Reverse Transcriptase-Polymerase Chain Reaction
Space Flight
Invertebrates
RNA extraction
030104 developmental biology
People and Places
Animal Studies
Caenorhabditis
lcsh:Q
Population Groupings
RNA
Helminth
Cosmic Radiation
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 10, p e0205852 (2018)
ISSN: 1932-6203
Popis: The distance and duration of human spaceflight missions is set to markedly increase over the coming decade as we prepare to send astronauts to Mars. However, the health impact of long-term exposure to cosmic radiation and microgravity is not fully understood. In order to identify the molecular mechanisms underpinning the effects of space travel on human health, we must develop the capacity to monitor changes in gene expression and DNA integrity in space. Here, we report successful implementation of three molecular biology procedures on board the International Space Station (ISS) using a miniaturized thermal cycler system and C. elegans as a model organism: first, DNA extraction-the initial step for any type of DNA analysis; second, reverse transcription of RNA to generate complementary DNA (cDNA); and third, the subsequent semi-quantitative PCR amplification of cDNA to analyze gene expression changes in space. These molecular procedures represent a significant expansion of the budding molecular biology capabilities of the ISS and will permit more complex analyses of space-induced genetic changes during spaceflight missions aboard the ISS and beyond.
Databáze: OpenAIRE
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