High prevalence of pfmdr1 N86Y and Y184F mutations in Plasmodium falciparum isolates from Bioko island, Equatorial Guinea
| Autor: | Santiago-m Monte-Nguba, Juan Urbano Monsuy Eyi, Min Lin, Rocio Apicante Matesa, Dong-De Xie, Danjie Lu, Hui Yang, Jiang-Tao Chen, Hui-Tian Yang, Maximo Miko Ondo Obono, Jian Li, Carlos Sala Ehapo, Li-Ye Yang |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Adolescent Short Communication Plasmodium falciparum Drug Resistance Mutation Missense Drug resistance medicine.disease_cause Microbiology Polymerase Chain Reaction law.invention Antimalarials Young Adult Gene Frequency law parasitic diseases medicine Parasite hosting Humans Malaria Falciparum Child Allele frequency Polymerase chain reaction Aged Aged 80 and over Mutation biology Haplotype Public Health Environmental and Occupational Health Infant General Medicine Sequence Analysis DNA DNA Protozoan Middle Aged biology.organism_classification medicine.disease Virology Infectious Diseases Child Preschool Parasitology Female Guinea Mutant Proteins Multidrug Resistance-Associated Proteins Malaria |
| Popis: | Drug resistance against Plasmodium falciparum has been recognized as the crucial obstacle to curbing mortality and morbidity from malaria. To investigate the distribution and pattern of multidrug resistance 1 (pfmdr1) gene polymorphisms in P. falciparum, isolates collected from the malaria high-endemic Bioko Island, Equatorial Guinea.Blood samples were collected from 217 patients with P. falciparum malaria during rainy season in 2012 on Bioko Island. These samples were extracted using Chelex to obtain parasite DNA. Nest-polymerase chain reaction (PCR) and sequencing were employed to detect mutations (N86Y, E130K, Y184F, S1034C, N1042D, V1109I, and D1246Y) and haplotypes in pfmdr1 gene.A total of 151 samples were successfully detected for pfmdr1 mutations from the 217 patients. Pfmdr1 mutations were found in 91·39% (138/151) P. falciparum isolates. However, no mutation at 130 and 1109 was identified from these samples. Four haplotypes coding 86, 184, 1034, 1,042, and 1,246 were found including NYSND, YYSND, NFSND, and YFSND, which accounted for 8·61% (13/151), 2·65% (4/151), 29·80% (45/151), and 58·94% (89/151), respectively.Our results exhibited hypersensitivity to lumefantrine (LU) and mefloquine (MQ) and resistance to chloroquine (CQ) and amodiaquine (AQ) in P. falciparum isolates from Bioko Island. This information will be useful for anti-malarial drug policy in Equatorial Guinea. |
| Databáze: | OpenAIRE |
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