Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells
| Autor: | Martin Merkel, Thomas Dobner, Kathryn J. Moore, May Brundert, F. Rinninger, Rajasekhar Ramakrishnan, Antonella Carambia, Peter Groitl, Johannes Herkel, Joerg Heeren |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
CD36 Antigens
medicine.medical_specialty HDL Apolipoprotein B CD36 Immunoblotting Stimulation QD415-436 liver Biochemistry Cell Line Mice chemistry.chemical_compound Endocrinology In vivo Internal medicine parasitic diseases medicine Animals selective Scavenger receptor Cells Cultured Research Articles Mice Knockout biology Cholesterol nutritional and metabolic diseases Biological Transport Cell Biology adrenal chemistry Cell culture cholesteryl ester Hepatocytes biology.protein Cholesteryl ester lipids (amino acids peptides and proteins) Cholesterol Esters Lipoproteins HDL |
| Zdroj: | Journal of Lipid Research, Vol 52, Iss 4, Pp 745-758 (2011) |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.m011981 |
| Popis: | The mechanisms of HDL-mediated cholesterol transport from peripheral tissues to the liver are incompletely defined. Here the function of scavenger receptor cluster of differentiation 36 (CD36) for HDL uptake by the liver was investigated. CD36 knockout (KO) mice, which were the model, have a 37% increase (P = 0.008) of plasma HDL cholesterol compared with wild-type (WT) littermates. To explore the mechanism of this increase, HDL metabolism was investigated with HDL radiolabeled in the apolipoprotein (125I) and cholesteryl ester (CE, [3H]) moiety. Liver uptake of [3H] and 125I from HDL decreased in CD36 KO mice and the difference, i. e. hepatic selective CE uptake ([3H]125I), declined (–33%, P = 0.0003) in CD36 KO compared with WT mice. Hepatic HDL holo-particle uptake (125I) decreased (–29%, P = 0.0038) in CD36 KO mice. In vitro, uptake of 125I-/[3H]HDL by primary liver cells from WT or CD36 KO mice revealed a diminished HDL uptake in CD36-deficient hepatocytes. Adenovirus-mediated expression of CD36 in cells induced an increase in selective CE uptake from HDL and a stimulation of holo-particle internalization. In conclusion, CD36 plays a role in HDL uptake in mice and by cultured cells. A physiologic function of CD36 in HDL metabolism in vivo is suggested. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|