Protective effects of ulinastatin against ischemia-reperfusion injury
| Autor: | Kaoru Taira, Hirofumi Tomori, Yoshihiro Muto, Toure Mamadi, Masayuki Shiraishi, Yukihiro Okuhama, Takao Higa |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Neutrophils medicine.medical_treatment Neutrophile Ischemia In Vitro Techniques chemistry.chemical_compound Internal medicine Medicine Animals Rats Wistar Glycoproteins Chemotherapy biology business.industry Vascular disease Ulinastatin medicine.disease In vitro Rats Microscopy Electron Endocrinology chemistry Liver Enzyme inhibitor Reperfusion Injury Immunology biology.protein Surgery business Trypsin Inhibitors Reperfusion injury Liver Circulation |
| Zdroj: | The Journal of surgical research. 82(1) |
| ISSN: | 0022-4804 |
| Popis: | We investigated the protective effect of urinary trypsin inhibitor (ulinastatin: UTI) in vitro, in relation to the neutrophil activity in hepatic ischemia/reperfusion (I/R) injury. The rat liver was removed and preserved in cold Ringer's lactate solution for 60 min, followed by 120 min of reperfusion with oxygenated perfusate. The rats were divided into four groups (n = 8 in each group). The livers were perfused with Krebs-Henseleit (K-H) solution containing no additives in group 1, 50,000 U/kg of UTI in group 2, 3.5 x 10(6) of neutrophils in group 3, and both neutrophils and UTI in group 4. In group 3, the AST and ALT levels were always higher than those in other three groups at any point evaluated (P < 0.01) and the LDH levels were observed to be significantly higher than those in other three groups at 0, 5, 10, 60, and 90 min after reperfusion (P < 0. 01). These increase were suppressed by additional pretreatment with UTI in group 4. The bile flow during reperfusion was significantly suppressed in group 3 compared to that of group 4, at both 30 (P < 0. 01) and 60 (P < 0.05) min after reperfusion. The MPO activity after reperfusion in group 3 also significantly increased compared to other three groups (P < 0.01). These data thus suggest that UTI ameliorated the ischemia/reperfusion injury in vitro by inhibiting of neutrophil accumulation in the postischemic liver. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect