Targeting of Polycomb Repressive Complex 2 to RNA by Short Repeats of Consecutive Guanines
| Autor: | Daniel T. Youmans, Karen J. Goodrich, Stuart K. Archer, Chen Davidovich, Anne R. Gooding, Haroon Naeem, Richard D. Paucek, Xueyin Wang, Thomas R. Cech |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Guanine macromolecular substances Biology Transfection 03 medical and health sciences Structure-Activity Relationship Nucleosome Humans Enhancer of Zeste Homolog 2 Protein Binding site Nucleotide Motifs Molecular Biology Gene Genetics Binding Sites Intron Polycomb Repressive Complex 2 RNA Cell Biology Non-coding RNA Chromatin Cell biology Nucleosomes RNA silencing 030104 developmental biology HEK293 Cells Gene Expression Regulation Nucleic Acid Conformation Protein Binding |
| Zdroj: | Molecular cell. 65(6) |
| ISSN: | 1097-4164 |
| Popis: | Polycomb repressive complex 2 (PRC2) is a histone methyltransferase that trimethylates H3K27, a mark of repressed chromatin. Mammalian PRC2 binds RNA promiscuously, with thousands of target transcripts in vivo. But what does PRC2 recognize in these RNAs? Here we show that purified human PRC2 recognizes G > C,U ≫ A in single-stranded RNA and has a high affinity for folded guanine quadruplex (G4) structures but little binding to duplex RNAs. Importantly, G-tract motifs are significantly enriched among PRC2-binding transcripts in vivo. DNA sequences coding for PRC2-binding RNA motifs are enriched at PRC2-binding sites on chromatin and H3K27me3-modified nucleosomes. Collectively, the abundance of PRC2-binding RNA motifs rationalizes the promiscuous RNA binding of PRC2, and their enrichment at Polycomb target genes provides a means for RNA-mediated regulation. |
| Databáze: | OpenAIRE |
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