Peptide mimics of hapten DNP: The effect of affinity of anti-DNP monoclonal antibodies for the selection of phage-displayed mimotopes
| Autor: | Colin R. Howard, Atila T. Kalaycioglu, P.H. Russell |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Phage display
medicine.drug_class Molecular Sequence Data Immunology Antibody Affinity Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Peptide Biopanning Monoclonal antibody Epitopes Mice Antigen Peptide Library medicine Animals Immunology and Allergy Amino Acid Sequence DNA Primers chemistry.chemical_classification Base Sequence biology Chemistry Molecular Mimicry Antibodies Monoclonal Molecular biology Amino acid biology.protein Rabbits Antibody Peptides Haptens Hapten Dinitrophenols |
| Zdroj: | Journal of Immunological Methods. 366:36-42 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/j.jim.2011.01.004 |
| Popis: | Biopanning of two linear (6- and 15-mer) and two constrained (10- and 17-mer) phage-displayed peptide libraries with two anti-DNP monoclonal antibodies (mAbs) selected seven unique peptide sequences using only the low affinity anti-DNP monoclonal antibody. The selected peptides contained two of 6, one of 10, two of 15 and two of 17 amino acids in length. They were all rich in hydrophobic residues. Both 15-mer peptides had antigenic regions of eight amino acids as revealed by a spot scan assay. Two of the 17-mer and one of the 10-mer peptides displayed on phage competed with free DNP for the low affinity anti-DNP mAb. These findings highlight (i) the selective power of phage displayed peptide libraries to identify peptides that mimic the shape of a small hapten molecule such as DNP, (ii) the possible preferential bias of phage libraries towards low affinity antibodies, (iii) the importance of using a panel of phage libraries for selecting peptide mimics. |
| Databáze: | OpenAIRE |
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