Fully inkjet-printed glucose assay fabricated on highly porous pigment coating
| Autor: | Risto Koivunen, Roger Bollström, Patrick A.C. Gane, Eveliina Jutila |
|---|---|
| Přispěvatelé: | Department of Bioproducts and Biosystems, Omya AG, Aalto-yliopisto, Aalto University |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
02 engineering and technology
engineering.material 01 natural sciences Polyvinyl alcohol Horseradish peroxidase chemistry.chemical_compound Highly porous coating Coating Paper microfluidics Materials Chemistry Glucose oxidase Cellulose Alkyl chemistry.chemical_classification Chromatography biology Microfluidic device 010401 analytical chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Enzyme assay 0104 chemical sciences Electronic Optical and Magnetic Materials chemistry Reagent biology.protein engineering Glucose assay 0210 nano-technology |
| Popis: | A simple paper-based microfluidic device, fabricated on a highly porous coating consisting of functionalised calcium carbonate (FCC) pigment and a microfibrillated cellulose (MFC) and/or polyvinyl alcohol (PVOH) binder, was developed for glucose detection. Both the hydrophobic patterns, consisting of alkyl ketene dimer (AKD), and the enzyme ink, consisting of glucose oxidase (GOx), horseradish peroxidase (HRP) and an indicator containing 4-aminoantipyrine (4-AAP), and sodium 3,5-dichloro-2-hydroxy-benzenesulphonic acid (DHBS) were inkjet-printed. Multiple coating formulations were tested using two indicators, potassium iodide (KI) and 4-AAP/DHBS, to find the optimal formulation with regard to detection sensitivity and assay stability. Higher binder concentrations increased the enzyme activity, especially in the case of PVOH. Two coatings, containing either solely MFC or both MFC and PVOH were ultimately chosen for the glucose assay experiments. For the assays, a 42 nl volume of buffered enzyme solution, containing a total of 5.04 mU GOx and approximately 1.01 mU HRP, in combination with 4 mM 4-AAP and 8 mM DHBS, was printed, and a 1 µl glucose solution, made up over a range of concentrations, was applied onto the samples to evaluate the response. A good linearity was achieved between detection and glucose concentration between 0.1 and 0.6 mM with both coatings. The assays remained stable for four weeks when stored at − 20 °C. The results show that the functional coated substrates offer a viable alternative to cellulose-based substrates for microfluidic applications enabling the use of small reagent and sample volumes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|