An improved system for exposure of cultured mammalian cells to gaseous compounds in the chromosomal aberration assay
| Autor: | Shoji Fukushima, Toshiaki Sasaki, Toshie Sugiyama, Heihachiro Arito, Masumi Asakura, Taijiro Matsushima |
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| Rok vydání: | 2008 |
| Předmět: |
Ethyl Chloride
Health Toxicology and Mutagenesis Cell Culture Techniques Vinyl Chloride Chlorodifluoromethane Biology Propyne Chloride Vinyl chloride Chinese hamster Polyploidy chemistry.chemical_compound Bromide Cricetinae Butadienes Genetics medicine Animals Lung Chromosome Aberrations Air Pollutants Chromatography Mutagenicity Tests biology.organism_classification Hydrocarbons Brominated chemistry Cell culture Alkynes Methyl Chloride Female Gases Chlorofluorocarbons Methane medicine.drug |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 652:122-130 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/j.mrgentox.2008.01.004 |
| Popis: | A gas exposure system using rotating vessels was improved for exposure of cultured mammalian cells to gaseous compounds in the chromosomal aberration assay. This system was composed of 12 square culture vessels, a device for preparation of air containing test gas, and positive and negative control gases at target concentrations and for supplying these gases to the culture vessels, and a roller apparatus in an incubator. Chinese hamster lung cells (CHL/IU) were grown on one side of the inner surface of the square culture vessel in the MEM medium. Immediately prior to exposure, the medium was changed to the modified MEM. Air in the culture vessel was replaced with air containing test gas, positive or negative control gas. Then, the culture vessels were rotated at 1.0 rpm. The monolayered culture cells were exposed to test gas during about 3/4 rotation at upper positions and alternatively immersed into the culture medium during about 1/4 rotation at lower positions. This system allowed the chromosomal aberration assay simultaneously at least at three different concentrations of a test gas together with positive and negative control gases with and without metabolic activations, and duplicate culture at each exposure concentration. Seven gaseous compounds, 1,3-butadiene, chlorodifluoromethane, ethyl chloride, methyl bromide, methyl chloride, propyne, and vinyl chloride, none of which has been tested to date, were tested on CHL/IU for the chromosomal aberration assay using this gas exposure system. All the compounds except chlorodifluoromethane showed positive responses of the structural chromosomal aberrations, whereas polyploidy was not induced by any of these gases. This improved gas exposure system proved to be useful for detecting chromosomal aberrations of gaseous compounds. |
| Databáze: | OpenAIRE |
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