Dysbiosis of fecal microbiota in cats with naturally occurring and experimentally induced Tritrichomonas foetus infection

Autor: Stephen H. Stauffer, Jody L. Gookin, Metzere Bierlein, Barry A. Hedgespeth, M. Andrea Azcarate-Peril
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Artificial Gene Amplification and Extension
Tritrichomonas foetus
medicine.disease_cause
Pathology and Laboratory Medicine
Polymerase Chain Reaction
Feces
Mice
Medicine and Health Sciences
Helicobacter
Tritrichomonas
Mammals
Multidisciplinary
CATS
Microbiota
Eukaryota
Genomics
Bacterial Pathogens
Diarrhea
Medical Microbiology
Vertebrates
Medicine
medicine.symptom
Anatomy
Pathogens
Research Article
Colon
Science
Gastroenterology and Hepatology
Microbial Genomics
Biology
Research and Analysis Methods
Microbiology
Fusobacteria
Signs and Symptoms
medicine
Genetics
Animals
Microbiome
Molecular Biology Techniques
Molecular Biology
Microbial Pathogens
Protozoan Infections
Bacteria
Gut Bacteria
Organisms
Biology and Life Sciences
biology.organism_classification
medicine.disease
Gastrointestinal Tract
Disease Models
Animal
Amniotes
Cats
Dysbiosis
Trichomonas vaginalis
Clinical Medicine
Zoology
Digestive System
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 2, p e0246957 (2021)
ISSN: 1932-6203
Popis: The protozoal pathogen Tritrichomonas foetus infects the colon of domestic cats and is a major cause of chronic colitis and diarrhea. Treatment failure is common, but antibiotics may improve clinical signs in a subset of cats, leading researchers to question involvement of the colonic microbiota in disease pathogenesis. Studies performed in women with venereal Trichomonas vaginalis infections have revealed that dysbiosis of host microbiota contributes to pathogenicity with similar findings also found in mice with intestinal Tritrichomonas musculis The aim of this study was to characterize differences in the fecal microbiota of cats with and without naturally occurring T. foetus infection and in a group of kittens prior to and after experimentally induced infection. Archived fecal DNA from cats undergoing testing for T. foetus infection (n = 89) and experimentally infected kittens (n = 4; at pre-, 2 weeks, and 9 weeks post-infection) were analyzed by sequencing of 16S rRNA genes. Amongst the naturally infected population, the genera Megamonas and Helicobacter were significantly increased in prevalence and abundance in cats testing positive for T. foetus infection. In the group of four experimentally infected kittens, fecal samples post-infection had significantly lower abundance of genus Dialister and Megamonas and greater abundance of the class Betaproteobacteria and family Succinivibrionaceae. We hypothesize that T. foetus promotes dysbiosis by competition for fermentable substrates used by these bacteria and that metabolic byproducts may contribute to the pathogenesis of colonic inflammation and diarrhea. Future studies are warranted for the measurement of fecal concentrations of microbial and protozoal metabolites in cats with T. foetus infection for the identification of potential therapeutic targets.
Databáze: OpenAIRE
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