Effects of Monochromatic Illumination with LEDs Lights on the Growth and Photosynthetic Performance of
| Autor: | Evagelina Korozi, Iordanis Chatzipavlidis, Giorgos Markou, Alexandros Diamantis, Io Kefalogianni, Vasiliki Tsagou |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Chlorophyll b Chlorophyll a Photosystem II Heterotroph Plastoquinone single-cell protein Plant Science glycerol Auxenochlorella Photosynthesis 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound mixotrophy 010608 biotechnology Food science monochromatic illumination Ecology Evolution Behavior and Systematics 030304 developmental biology photosynthetic performance 0303 health sciences Ecology biology microalgae Botany biology.organism_classification chemistry QK1-989 Mixotroph |
| Zdroj: | Plants, Vol 10, Iss 799, p 799 (2021) Plants Volume 10 Issue 4 |
| ISSN: | 2223-7747 |
| Popis: | This study examined the effects of monochromatic illumination (blue, red, green and yellow) employing light-emitting diodes (LEDs), trophic conditions (photoautotrophic and mixotrophic), and nitrogen availability (high and low peptone concentration) on the growth and biochemical composition of Auxenochlorella protothecoides. The results revealed that mixotrophic conditions did not favor A. protothecoides, giving lower growth rates compared to heterotrophy (dark conditions). However, mixotrophy gave significantly higher growth rates compared to photoautotrophy. The best light wavelengths for mixotrophic cultivation were that of white and red. In all cases investigated in this study, high peptone concentration (4 g/L) resulted in decreased growth rates. Regarding the biochemical composition of A. protothecoides, the strongest effect, irrespective of trophic conditions, was caused by nitrogen availability (peptone concentration). Specifically, at nitrogen replete conditions (4 g/L peptone), biomass was rich in proteins (32–67%), whereas under deplete conditions (0.5 g/L peptone), A. protothecoides accumulated mainly carbohydrates (up to 56%). Mixotrophic conditions generally favored higher carbohydrate content, whereas photoautotrophic conditions favored higher protein content. The different illumination spectra did not have any clear effect on the biochemical composition (metabolites content), except that, in all trophic conditions, the use of the green spectrum resulted in higher chlorophyll b content. Chlorophyll a fluorescence studies revealed that the trophic conditions and the high peptone concentrations impacted the photosystem II (PSII) performance, and also affected plastoquinone re-oxidation kinetics and the heterogeneity of the PSII reaction centers. |
| Databáze: | OpenAIRE |
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