Channel characteristics of VDAC-3 from Arabidopsis thaliana

Autor: Geneviève Ephritikhine, Rémi Peyronnet, Alexandre Ghazi, Hélène Barbier-Brygoo, Jean-Michel Betton, Jean-Marie Frachisse, Catherine Berrier
Přispěvatelé: Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Microbiologie structurale - Structural Microbiology (Microb. Struc. (UMR_3528 / U-Pasteur_5)), Université Paris Diderot - Paris 7 (UPD7)-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This work was funded by the Centre National de la Recherche Scientifique and by Agence Nationale de la Recherche Grant BLAN06-2-135035, Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences de Paris-Saclay ( Neuro-PSI ), Université Paris-Sud - Paris 11 ( UP11 ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Sud - Paris 11 ( UP11 ), Microbiologie structurale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Institut des Neurosciences de Paris-Saclay (Neuro-PSI), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2015
Předmět:
Gene isoform
MESH : Escherichia coli
Voltage-dependent anion channel
MESH : Recombinant Proteins
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Lipid Bilayers
Biophysics
MESH: Arabidopsis Proteins
Protein Engineering
Biochemistry
MESH : Arabidopsis Proteins
Inclusion bodies
[ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
MESH: Recombinant Proteins
MESH : Cell-Free System
[ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Arabidopsis
MESH: Cell-Free System
Escherichia coli
Voltage-Dependent Anion Channels
Arabidopsis thaliana
MESH: Electrophysiological Phenomena
Lipid bilayer
Molecular Biology
Cell-Free System
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
biology
Arabidopsis Proteins
MESH : Voltage-Dependent Anion Channels
MESH: Escherichia coli
MESH: Lipid Bilayers
MESH: Voltage-Dependent Anion Channels
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Cell Biology
Protein engineering
Plant cell
biology.organism_classification
Recombinant Proteins
Electrophysiological Phenomena
MESH: Protein Engineering
MESH : Protein Engineering
[ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MESH : Electrophysiological Phenomena
biology.protein
MESH : Lipid Bilayers
Zdroj: Biochemical and Biophysical Research Communications
Biochemical and Biophysical Research Communications, Elsevier, 2015, 459 (1), pp.24-8. ⟨10.1016/j.bbrc.2015.02.034⟩
Biochemical and Biophysical Research Communications, 2015, 459 (1), pp.24-8. ⟨10.1016/j.bbrc.2015.02.034⟩
Biochemical and Biophysical Research Communications, Elsevier, 2015, 459 (1), pp.24-8. 〈10.1016/j.bbrc.2015.02.034〉
ISSN: 0006-291X
1090-2104
DOI: 10.1016/j.bbrc.2015.02.034
Popis: International audience; Four different isoforms of the Voltage-Dependent Anion Channel (VDAC) have been identified in Arabidopsis plant cells. The electrophysiological characteristics of several VDAC channels from animal as well as plant cells are well documented, but those of this model plant are unknown. One isoform, AtVDAC-3 was obtained either directly by cell-free synthesis or produced in Escherichia coli, as inclusion bodies, and re-natured. An electrophysiological study of the purified proteins in planar lipid bilayers showed that both methods yielded proteins with similar channel activity. The characteristics of AtVDAC-3 are that of a bona fide VDAC-like channel.
Databáze: OpenAIRE
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