Role of EspB in Experimental Human Enteropathogenic Escherichia coli Infection
| Autor: | Akio Abe, James P. Nataro, Stephen P. James, Marcelo B. Sztein, Genevieve Losonsky, George T. Fantry, B. Brett Finlay, Barry P. McNamara, Carol O. Tacket, Michael S. Donnenberg, Myron M. Levine |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Diarrhea Adolescent Biopsy Immunology Virulence Biology Escherichia coli O157 medicine.disease_cause Microbiology Interferon-gamma Immune system Double-Blind Method Antigen Immunity Escherichia coli medicine Humans Secretion Enteropathogenic Escherichia coli Escherichia coli Infections Immunity Cellular Microvilli Escherichia coli Proteins Vaccination Bacterial Infections Antibodies Bacterial Virology Jejunum Infectious Diseases Humoral immunity Parasitology Bacterial Outer Membrane Proteins |
| Zdroj: | Infection and Immunity. 68:3689-3695 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Enteropathogenic Escherichia coli (EPEC), a leading cause of diarrhea among infants in developing countries, induces dramatic alterations in host cell architecture that depend on a type III secretion system. EspB, one of the proteins secreted and translocated to the host cytoplasm via this system, is required for numerous alterations in host cell structure and function. To determine the role of EspB in virulence, we conducted a randomized, double-blind trial comparing the ability of wild-type EPEC and an isogenic Δ espB mutant strain to cause diarrhea in adult volunteers. Diarrhea developed in 9 of 10 volunteers who ingested the wild-type strain but in only 1 of 10 volunteers who ingested the Δ espB mutant strain. Marked destruction of the microvillous brush border adjacent to adherent organisms was observed in a jejunal biopsy from a volunteer who ingested the wild-type strain but not from two volunteers who ingested the Δ espB mutant strain. Humoral and cell-mediated immune responses to EPEC antigens were stronger among recipients of the wild-type strain. In addition, four of the volunteers who ingested the wild-type strain had lymphoproliferative responses to EspB. These results demonstrate that EspB is a critical virulence determinant of EPEC infections and suggest that EspB contributes to an immune response. |
| Databáze: | OpenAIRE |
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