Emergence of extended-spectrum β-lactamase (ESBL) and/or carbapenemase producing Enterobacteriaceae (CPE) and their antimicrobial resistance
| Autor: | Z Hubenakova, Hana Drahovská, Aneta Lichvariková, J Koren, E Ozaee, Barbora Markusková |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Economics and Econometrics Avibactam Tigecycline Microbial Sensitivity Tests Fosfomycin Meropenem beta-Lactamases Microbiology chemistry.chemical_compound Antibiotic resistance Bacterial Proteins Enterobacteriaceae Drug Resistance Bacterial polycyclic compounds Materials Chemistry Media Technology medicine Escherichia coli Humans Aged biology Enterobacteriaceae Infections Forestry Enterobacter biochemical phenomena metabolism and nutrition Middle Aged bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Klebsiella pneumoniae Carbapenem-Resistant Enterobacteriaceae chemistry Amikacin Colistin bacteria medicine.drug |
| Zdroj: | Bratislavske lekarske listy. 120(12) |
| ISSN: | 0006-9248 |
| Popis: | Objectives We focused on detecting the most frequent resistance mechanisms in selected multidrug-resistant (MDR) strains and determining their antimicrobial resistance. Background MDR pathogens pose urgent public health threat due to limited treatment options, rigorous control measures and significant mortality. Methods We confirmed extended-spectrum β-lactamase (ESBL) and carbapenemase producing Enterobacteriaceae through guidelines, as well following β-lactamases: AmpC by cloxacillin, class A carbapenemase with phenylboronic acid, class B metallo-β-lactamase with ethylenediaminetetraacetic acid. Multilocus sequence typing was used to investigate 20 Escherichia coli strains. Results Overall 205 mostly ESBL Escherichia coli demonstrated resistance against amikacin (4.7 %), tigecycline (1.2 %), and no resistance to ceftazidime/avibactam, meropenem, nitrofurantoin and fosfomycin. Out of 41 Klebsiella species (spp.), 37 (90.2 %) showed carbapenemase activity, 13 (35.1 %) of class A and 24 (64.9 %) of class B. Resistance was following: meropenem 66.7 %, tigecyclin 10.2 % and colistin 0 %. From Enterobacter spp. 21 strains, 14 (66.7 %) were ESBL, 5 produced ESBL and/or AmpC and 2 were MDR. We ascertained 14 (70 %) E. coli sequence type - ST131. Conclusions The study revealed various resistance mechanisms in concert with different agents and association of specific ST131 within E. coli. These characteristics considerably contribute to emergence of antimicrobial resistance (Tab. 4, Ref. 30). |
| Databáze: | OpenAIRE |
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