Elemental bio-imaging of melanoma in lymph node biopsies
| Autor: | Philip Doble, Richard A. Scolyer, Fred Fryer, Dominic J. Hare, Rudolf Grimm, John F. Thompson, Christine Austin, Fiona Burger, Brian Reedy |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Haematoxylin Biochemistry Mass Spectrometry Analytical Chemistry Bio imaging chemistry.chemical_compound Electrochemistry medicine Biomarkers Tumor Environmental Chemistry Humans Lymph node Melanoma Spectroscopy Eosin Chemistry Sentinel Lymph Node Biopsy Capsule Phosphorus medicine.disease Radiology and Organ Imaging Staining Trace Elements medicine.anatomical_structure Tumor Markers Biological Lymphatic Metastasis Lymph |
| Popis: | The spatial distribution of trace elements in human lymph nodes partially infiltrated by melanoma cells was determined by elemental bio-imaging. Imaging of 31P within the nodal capsule and normal lymph node tissue showed a clear demarcation of the tumour boundary, with a significant decrease in relative 31P concentration within the tumour. The location of the tumour boundary was confirmed by haematoxylin and eosin staining of serial sections and observation by light microscopy. Further enhancement of the tumour boundary was achieved by imaging the 31P/34S ratio. 31P/66Zn ratio images showed a decreasing ratio beyond the tumour boundary that extended into peritumour normal lymph node tissue. © The Royal Society of Chemistry. |
| Databáze: | OpenAIRE |
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