A Safety Evaluation of DAS181, a Sialidase Fusion Protein, in Rodents
| Autor: | Matthew Nieder, Laura M. Aschenbrenner, Fang Fang, GloriaMay Machado, Maria Hedlund, Bo In Choi, Beth Cecil, Seong-Kwi Kang, Jeffrey L. Larson |
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| Rok vydání: | 2011 |
| Předmět: |
Male
glycoprotein Pathology medicine.medical_specialty Anemia Recombinant Fusion Proteins Drug Evaluation Preclinical Neuraminidase Physiology Biology Toxicology Cell Line Rats Sprague-Dawley Mice Toxicity Tests medicine Animals Humans Cytotoxic T cell Respiratory system Lung Inhalation exposure Inhalation Exposure Mice Inbred BALB C inhalation desialylation sialidase DAS181 desialylated erythrocytes Alkaline Phosphatase Orthomyxoviridae medicine.disease Hemolysis Rats medicine.anatomical_structure sialic acid Hepatocytes parainfluenza Alkaline phosphatase Female influenza Safety Evaluation Respiratory tract |
| Zdroj: | Toxicological Sciences |
| ISSN: | 1096-0929 1096-6080 |
| Popis: | DAS181 is a novel inhaled drug candidate blocking influenza virus (IFV) and parainfluenza virus (PIV) infections through removal of sialic acid receptors from epithelial surface of the respiratory tract. To support clinical development, a 28-day Good Laboratory Practices inhalation toxicology study was conducted in Sprague-Dawley rats. In this study, achieved average daily doses based on exposure concentrations were 0.47, 0.90, 1.55, and 3.00 mg/kg/day of DAS181 in a dry powder formulation. DAS181 was well tolerated at all dose levels, and there were no significant toxicological findings. DAS181 administration did not affect animal body weight, food consumption, clinical signs, ophthalmology, respiratory parameters, or organ weight. Gross pathology evaluations were unremarkable. Histological examination of the lungs was devoid of pulmonary tissue damage, and findings were limited to mild and transient changes indicative of exposure and clearance of a foreign protein. DAS181 did not show any cytotoxic effects on human and animal primary cells, including hepatocytes, skeletal muscle cells, osteoblasts, or respiratory epithelial cells. DAS181 did not cause direct or indirect hemolysis. A laboratory abnormality observed in the 28-day toxicology study was mild and transient anemia in male rats at the 3.00 mg/kg dose, which is an expected outcome of enhanced clearance of desialylated red blood cells resulting from systemic exposure with DAS181. Another laboratory observation was a transient dose-dependent elevation in alkaline phosphatase (ALP), which can be attributed to reduced ALP clearance resulting from increased protein desialylation due to DAS181 systemic exposure. These laboratory parameters returned to normal at the end of the recovery period. |
| Databáze: | OpenAIRE |
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