GPER1 in sand rat epididymis: Effects of seasonal variations, castration and efferent ducts ligation
| Autor: | Xavier Bonnet, Farida Khammar, Souaâd Smaï, Rafik Menad, Thérèse Gernigon-Spychalowicz, Meriem Fernini, Elara Moudilou, Jean-Marie Exbrayat |
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| Přispěvatelé: | Small Vertebrates Reproduction, Laboratory of Research on Arid Areas [Algeria], Faculty of Sciences, Department of Natural and Life Sciences [Algeria], Algiers University I, Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université des Sciences et de la Technologie Houari Boumediene [Alger] (USTHB), Laboratory of General Biology, Catholic University of Lyon [France], University of Lyon, Institut National de la Recherche Agronomique (INRA)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty medicine.drug_class Sand rat Estrogen receptor Biology GPER1 Receptors G-Protein-Coupled 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Food Animals Western blot Internal medicine medicine Animals Castration Receptor Ligation Testosterone Epididymis medicine.diagnostic_test Efferent ducts General Medicine 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Receptors Estrogen chemistry Estrogen 030220 oncology & carcinogenesis [SDE]Environmental Sciences Animal Science and Zoology Seasons Season Gerbillinae Orchiectomy |
| Zdroj: | Animal Reproduction Science Animal Reproduction Science, Elsevier Masson, 2017, 183, pp.9-20. ⟨10.1016/j.anireprosci.2017.06.012⟩ |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2017.06.012 |
| Popis: | International audience; Estrogen plays a crucial role in regulating epididymal function and development. Estrogen signalingis mediated via two main receptors essentially involved in the genomic regulatingpathway: ERα and ERβ. Recent studies revealed the contribution of a novel estrogen receptorinvolved in the non-genomic pathway: GPER1. This receptor belongs to the family of seventransmembraneG-protein-coupled receptors and it triggers rapid cellular responses. Immunohistochemicalstudies and Western Blot analyses were performed to investigate the GPER1 expressionin the caput and cauda epididymis of free-ranging fat sand rats (Psammomys obesus)captured during the breeding and resting seasons. We also investigated the effect of castration(C), castration followed by testosterone treatment (C+ T), and ligation of the efferent ducts (L).During the breeding season, a marked positive GPER1 immunoreactivity was detected in thecytoplasm of principal cells and basal cells; this signal persisted during the resting season, attenuatedhowever, meanwhile the clear cells were not immuno-reactive. In C animals, the immuno-histochemical staining underwent nuclear translocation. In C +T animals, this responsebecame nuclear and cytoplasmic. In the L group, the expression of the GPER1 was mainly locatedin the cytoplasm of principal cells and in the nuclei of basal cells; the sperm was also immunepositivein the cauda epididymis. Western blot analysis showed that GPER1 has a molecularweight of 55 kDa in the caput and cauda epididymis during the breeding season, and it persistedduring the resting season in the caput epididymis with a decrease in the cauda epididymis. Theseresults suggest that GPER1 mediate a specific cellular estrogen signaling with marked differencesbetween the breeding and resting seasons. Experimental groups suggest that testosterone is involvedin the regulation of the expression of GPER1, in addition to other estrogen signalizationpathways. |
| Databáze: | OpenAIRE |
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