PEMT, Δ6 desaturase, and palmitoyldocosahexaenoyl phosphatidylcholine are increased in rats during pregnancy[S]
| Autor: | Daniel Chalil, David M. Mutch, Jason L. Elzinga, Juan J. Aristizabal Henao, Alan Chalil, Kristin A. Marks, Ken D. Stark, Daniel M.E. Lamontagne-Kam, Flavia Badoud, Alex P. Kitson |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty genetic structures FADS2 Phosphatidylethanolamine N-Methyltransferase QD415-436 Linoleoyl-CoA Desaturase fatty acids Biochemistry Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Endocrinology Pregnancy Internal medicine Phosphatidylcholine tandem mass spectrometry medicine Animals Research Articles phospholipids nutrition/lipids 2. Zero hunger chemistry.chemical_classification Fetus omega-3 fatty acids Chemistry Fatty acid food and beverages Cell Biology Metabolism medicine.disease 3. Good health Rats 030104 developmental biology Phosphatidylethanolamine N-methyltransferase Phosphatidylcholines Female lipids (amino acids peptides and proteins) microarray Polyunsaturated fatty acid |
| Zdroj: | Journal of Lipid Research, Vol 59, Iss 1, Pp 123-136 (2018) |
| ISSN: | 0022-2275 |
| Popis: | DHA is important for fetal neurodevelopment. During pregnancy, maternal plasma DHA increases, but the mechanism is not fully understood. Using rats fed a fixed-formula diet (DHA as 0.07% total energy), plasma and liver were collected for fatty acid profiling before pregnancy, at 15 and 20 days of pregnancy, and 7 days postpartum. Phosphatidylethanolamine methyltransferase (PEMT) and enzymes involved in PUFA synthesis were examined in liver. Ad hoc transcriptomic and lipidomic analyses were also performed. With pregnancy, DHA increased in liver and plasma lipids, with a large increase in plasma DHA between day 15 and day 20 that was mainly attributed to an increase in 16:0/DHA phosphatidylcholine (PC) in liver (2.6-fold) and plasma (3.9-fold). Increased protein levels of Δ6 desaturase (FADS2) and PEMT at day 20 and increased Pemt expression and PEMT activity at day 15 suggest that during pregnancy, both DHA synthesis and 16:0/DHA PC synthesis are upregulated. Transcriptomic analysis revealed minor changes in the expression of genes related to phospholipid synthesis, but little insight on DHA metabolism. Hepatic PEMT appears to be the mechanism for increased plasma 16:0/DHA PC, which is supported by increased DHA biosynthesis based on increased FADS2 protein levels. |
| Databáze: | OpenAIRE |
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